Interleukin 1- and 2-like activities in the dog
- 31 July 1984
- journal article
- research article
- Published by Elsevier BV in Veterinary Immunology and Immunopathology
- Vol. 6 (3-4), 261-271
- https://doi.org/10.1016/0165-2427(84)90052-7
Abstract
Canine adherent and non-adherent peripheral blood leukocytes and spleen cells were examined for their ability to produce soluble factors with Interleukin 1- and 2- (IL-1 and IL-2) like activities. For this purpose, three conventional assay systems were used: (a) proliferation on an IL-2-dependent murine cytotoxic T-lymphocyte cell line, (b) enhancement of PHA-induced murine thymocyte proliferation and (c) proliferation of lectin-primed canine peripheral blood lymphocytes (PBL). Only the latter two types of cells respond to IL-1, whereas all three types respond to IL-2. Both types of factors were produced and the kinetics of their release/production were found to be identical to those of human PBL. Results suggested that species-related differences existed. Canine interleukin-containing supernatants had higher titers than murine interleukin-containing supernatants when analyzed on canine lymphocytes, and the reverse was found if murine target cells were used.Keywords
This publication has 21 references indexed in Scilit:
- A monoclonal antibody that appears to recognize the receptor for human T-cell growth factor; partial characterization of the receptorNature, 1982
- Assessment of interleukin 1 and interleukin 2 effects on cycling and noncycling murine thymocytesCellular Immunology, 1982
- Canine lymphocyte cultures in vitro: Evaluation of peripheral blood lymphocyte response to mitogensVeterinary Immunology and Immunopathology, 1982
- Bovine costimulator. II. Generation and maintenance of a bovine costimulator-dependent bovine lymphoblastoid cell lineVeterinary Immunology and Immunopathology, 1982
- Bovine costimulator. I. Production kinetics, partial purification, and quantification in serum-free Iscove's mediumVeterinary Immunology and Immunopathology, 1982
- Human lymphocyte proliferation II. Formation of activated (G1) cellsEuropean Journal of Immunology, 1982
- Analysis of the in vitro activation and proliferation process in lymphocytes deriving from canine thymus and mesenteric lymphnodeVeterinary Immunology and Immunopathology, 1981
- Relationship between production and release of lymphocyte-activating factor (interleukin 1) by murine macrophages: I. Effects of various agentsCellular Immunology, 1981
- Thymocyte activation by cytokines: Direct assessment of G0-G1 transition by flow-cytometryCellular Immunology, 1980
- Mechanism of T cell activation I. A screening of “step one” ligandsEuropean Journal of Immunology, 1980