ATP stimulates calcium influx in primary astrocyte cultures
- 30 December 1988
- journal article
- Published by Elsevier BV in Biochemical and Biophysical Research Communications
- Vol. 157 (3), 1410-1416
- https://doi.org/10.1016/s0006-291x(88)81032-5
Abstract
The effect of ATP and other purines on 45Ca uptake was studied in primary cultures of rat astrocytes. Treatment of the cells with ATP for 1 to 30 min brought about an increase in cellular 45Ca. Stimulation of calcium influx by ATP was investigated using a 90 sec exposure to 45Ca and over a concentration range of 0.1 nM to 3 mM; a biphasic dose-response curve was obtained with EC50 values of 0.3 nM and 9 uM, indicating the presence of low and high affinity purinergic binding sites. Similar levels of 45Ca influx at 90 sec were observed with ATP, ADP and adenosine (all at 100 uM). Prior treatment of the cultures with LaCl3 blocked the purine-induced 45Ca influx. These findings indicate that one pathway for calcium entry in astrocytes involves purinergic receptor-operated, calcium channels.Keywords
This publication has 19 references indexed in Scilit:
- Calcium channels in thrombin-activated human platelet membraneNature, 1988
- Protein Kinase C in Primary Astrocyte Cultures: Cytoplasmic Localization and Translocation by a Phorbol EsterJournal of Neurochemistry, 1988
- A Spatial-Temporal Model of Cell ActivationScience, 1988
- Calmodulin Kinase II in Pure Cultured AstrocytesJournal of Neurochemistry, 1988
- Vasopressor peptides and depolarization stimulated Ca2+-entry into cultured vascular smooth muscleBiochemical and Biophysical Research Communications, 1987
- A novel receptor-operated Ca2+-permeable channel activated by ATP in smooth muscleNature, 1987
- Exogenous ATP-stimulated calcium uptake in isolated rat intestinal epithelial cellsLife Sciences, 1987
- Multiple Calcium Channels and Neuronal FunctionScience, 1987
- Studies and Perspectives of Protein Kinase CScience, 1986
- Voltage-Dependent Calcium Channels in Glial CellsScience, 1984