Genetic evidence for a novel thymidylate synthase in the halophilic archaeonHalobacterium salinarumand inCampylobacter jejuni
Open Access
- 1 October 2002
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 216 (1), 105-109
- https://doi.org/10.1111/j.1574-6968.2002.tb11422.x
Abstract
A search of the complete genome sequence of the halophilic archaeon Halobacterium salinarum failed to identify a gene homologous to the thymidylate synthase (thyA) gene present in the closely related Haloferax volcanii. To understand the source of thymidine synthesis in Hbt. salinarum, a genomic library of Hbt. salinarum was constructed and used to complement a Hfx. volcanii thyA deletion mutation. The Hbt. salinarum ORF that complemented the thyA mutation shares sequence homology with ORFs found in numerous microorganisms that lack a thyA gene, including the recently discovered thyX of Helicobacter pylori. We also show that a homolog of the Hbt. salinarum ORF is present in Campylobacter jejuni and is able to complement an Escherichia coli thyA mutant under oxygen-limiting conditions.Keywords
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