Identification of a novel bufavirus in domestic pigs by a viral metagenomic approach
- 1 July 2016
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 97 (7), 1592-1596
- https://doi.org/10.1099/jgv.0.000476
Abstract
Bufavirus is a single-stranded DNA virus belonging to the genus Protoparvovirus. This study reports the identification and characterization of a porcine bufavirus by a metagenomic approach, and a limited epidemiology investigation of bufavirus in 6 swine farms. A comparative genome analysis showed a similarity of 93% to a Hungarian porcine bufavirus. Bayesian and maximum likelihood analyses of genome sequences showed a close relationship of porcine bufaviruses to human and monkey bufaviruses. Molecular dating of the most recent common ancestors supported a recent introduction of bufaviruses into human and pig populations, respectively. A real-time PCR method was developed to screen 60 fecal samples for the porcine bufavirus DNA, and eight positive samples were found in two neighboring farms, suggesting a relatively low prevalence (13.3%). No direct transmission of porcine bufaviruses between two neighboring farms was found, suggesting that bufaviruses may have spread much widely in different geographical regions.Keywords
This publication has 19 references indexed in Scilit:
- Detection and genetic characterization of a novel parvovirus distantly related to human bufavirus in domestic pigsArchiv für die gesamte Virusforschung, 2016
- Identification of Bufavirus-1 and Bufavirus-3 in Feces of Patients with Acute Diarrhea, ChinaScientific Reports, 2015
- Bufavirus genotype 3 in Turkish children with severe diarrhoeaClinical Microbiology & Infection, 2015
- Bufavirus in fecal specimens of patients with and without diarrhea in ThailandArchiv für die gesamte Virusforschung, 2015
- The early spread and epidemic ignition of HIV-1 in human populationsScience, 2014
- The family ParvoviridaeArchiv für die gesamte Virusforschung, 2013
- Pathogenic Simian Immunodeficiency Virus Infection Is Associated with Expansion of the Enteric ViromeCell, 2012
- Bayesian Phylogenetics with BEAUti and the BEAST 1.7Molecular Biology and Evolution, 2012
- Detection of a Novel Astrovirus in Brain Tissue of Mink Suffering from Shaking Mink Syndrome by Use of Viral MetagenomicsJournal of Clinical Microbiology, 2010
- Insights into the Evolutionary History of an Emerging Livestock Pathogen: Porcine Circovirus 2Journal of Virology, 2009