Abundance and Activity of Methanotrophic Bacteria in Littoral and Profundal Sediments of Lake Constance (Germany)
- 1 January 2009
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 75 (1), 119-126
- https://doi.org/10.1128/aem.01350-08
Abstract
The abundances and activities of aerobic methane-oxidizing bacteria (MOB) were compared in depth profiles of littoral and profundal sediments of Lake Constance, Germany. Abundances were determined by quantitative PCR (qPCR) targeting the pmoA gene and by fluorescence in situ hybridization (FISH), and data were compared to methane oxidation rates calculated from high-resolution concentration profiles. qPCR using type I MOB-specific pmoA primers indicated that type I MOB represented a major proportion in both sediments at all depths. FISH indicated that in both sediments, type I MOB outnumbered type II MOB at least fourfold. Results obtained with both techniques indicated that in the littoral sediment, the highest numbers of methanotrophs were found at a depth of 2 to 3 cm, corresponding to the zone of highest methane oxidation activity, although no oxygen could be detected in this zone. In the profundal sediment, highest methane oxidation activities were found at a depth of 1 to 2 cm, while MOB abundance decreased gradually with sediment depth. In both sediments, MOB were also present at high numbers in deeper sediment layers where no methane oxidation activity could be observed.Keywords
This publication has 60 references indexed in Scilit:
- Molecular Ecology Techniques for the Study of Aerobic MethanotrophsApplied and Environmental Microbiology, 2008
- Methane oxidation at 55°C and pH 2 by a thermoacidophilic bacterium belonging to the Verrucomicrobia phylumProceedings of the National Academy of Sciences of the United States of America, 2008
- Comparison of Aerobic Methanotrophic Communities in Littoral and Profundal Sediments of Lake Constance by a Molecular ApproachApplied and Environmental Microbiology, 2007
- Use of 16S rRNA and rpoB Genes as Molecular Markers for Microbial Ecology StudiesApplied and Environmental Microbiology, 2007
- Probing the microenvironment of freshwater sediment macrofauna: Implications of deposit-feeding and bioirrigation for nitrogen cyclingLimnology and Oceanography, 2006
- Aerobic and anaerobic methanotrophs in the Black Sea water columnEnvironmental Microbiology, 2006
- A microbial consortium couples anaerobic methane oxidation to denitrificationNature, 2006
- Dynamics of Redox Changes of Iron Caused by Light–dark Variations in Littoral Sediment of a Freshwater LakeBiogeochemistry, 2005
- Methane Release through Resuspension of Littoral SedimentBiogeochemistry, 2005
- Basic local alignment search toolJournal of Molecular Biology, 1990