Protein‐coated poly(L‐lactic acid) fibers provide a substrate for differentiation of human skeletal muscle cells
- 6 April 2004
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 69A (3), 373-381
- https://doi.org/10.1002/jbm.a.30009
Abstract
Tissue engineering represents a potential method for repairing damaged skeletal muscle tissue. Extracellular matrix (ECM) proteins were evaluated for their ability to aid in cell attachment, whereas a poly(L-lactic acid) (PLLA) fiber scaffold was tested as a substrate for the differentiation of human skeletal muscle cells. In comparison to uncoated or gelatin-coated PLLA films, cell attachment increased significantly (p < 0.001) on PLLA films coated with ECM gel, fibronectin, or laminin. Myoblasts differentiated into multinucleated myofibers on ECM gel-coated PLLA fibers, and expressed muscle markers such as myosin and α-actinin. Oligonucleotide microarray analysis showed similar gene expression profiles for human skeletal muscle cells on ECM gel-coated PLLA fibers as to that observed for myofibers on tissue culture plates. Therefore, PLLA fibers coated with ECM proteins provide a scaffold for the development of skeletal muscle tissue for tissue engineering and cell transplantation applications. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 69A: 373–381, 2004Keywords
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