Budding of Rabies Virus Particles in the Absence of the Spike Glycoprotein
- 1 March 1996
- journal article
- research article
- Published by Elsevier BV in Cell
- Vol. 84 (6), 941-951
- https://doi.org/10.1016/s0092-8674(00)81072-7
Abstract
Budding of enveloped viruses from cellular membranes is believed to depend on the presence of transmembrane spike proteins interacting with cytoplasmic virus components. To address the mechanism of rhabdovirus budding, we generated rabies virus mutants deficient for the glycoprotein G or the G cytoplasmic tail. We found that spikeless rhabdovirus particles were released from cells infected with the G-deficient mutant, demonstrating that a viral surface protein is not required to drive the budding process. However, particle production is enhanced approximately 6-fold and 30-fold in the presence of tailless G or G, respectively. This reveals that G also possesses an intrinsic and independent exocytosis activity. We propose a model according to which efficient budding of rhabdoviruses is achieved by a concerted action of both core and spike proteins.Keywords
This publication has 42 references indexed in Scilit:
- Winston R. Maxted and the type specificity of group A streptococciJournal of Medical Microbiology, 2001
- Novel infectious particles generated by expression of the vesicular stomatitis virus glycoprotein from a self-replicating RNACell, 1994
- Macromolecular interactions in the assembly of HIV and other retrovirusesSeminars in Virology, 1994
- Membrane fusion activity, oligomerization, and assembly of the rabies virus glycoproteinVirology, 1991
- Fatty acylation of rabies virus proteinsVirology, 1991
- Molecular cloning and complete nucleotide sequence of the attenuated rabies virus SAD B19Virology, 1990
- Assembly and release of HIV-1 precursor Pr55gag virus-like particles from recombinant baculovirus-infected insect cellsCell, 1989
- Altered cytoplasmic domains affect intracellular transport of the vesicular stomatitis virus glycoproteinCell, 1983
- The Pseudotypic ParadoxJournal of General Virology, 1982
- Structure of the glycoprotein gene in rabies virusNature, 1981