Abstract
The influence of carbon dioxide at 1-55 atm on the germination of Clostridium sporogenes, C. perfringens and Bacillus cereus spores in a complex medium was studied. The germination studies at atmospheric pressure were done in the pH range 5.2-6.7. Controls at the same pH were done in 100% N2. CO2 at atmospheric pressure (1 atm) inhibited the spore germination of B. cereus spores but strongly enhanced the germination rate of those of the clostridia. Spore germination of C. sporogenes and C. perfringens was inhibited completely at 10 atm and at 25 atm, respectively. The germination rate in CO2 or N2 was generally higher at pH 6.7 than at 5.2-6.0.