Distinct populations of cells in the adult dentate gyrus undergo mitosis or apoptosis in response to adrenalectomy
- 20 May 1996
- journal article
- research article
- Published by Wiley in Journal of Comparative Neurology
Abstract
Granule neurons of the rat dentate gyrus are born in adulthood as well as during development. Apoptotic cell death also occurs normally in this population throughout the life of the rat. Removal of adrenal steroids results in both increased production and increased degeneration of dentate gyrus granule cells. In order to determine whether the age of a cell affects its response to adrenalectomy (ADX), the numbers of dentate gyrus cells of different ages were assessed following ADX or sham operation. Older cells, i.e., those labeled with the thymidine analog bromodeoxyuridine (BrdU) on postnatal day (P) 6, were reduced in number following ADX on P60, and some had the morphologic characteristics of degenerating cells, indicating that significant numbers of mature cells die in response to ADX. In contrast, the number of younger cells, labeled with 3H‐thymidine or BrdU in adulthood, 24 hours or 2 weeks before ADX, did not decrease, suggesting that these less mature cells do not die following ADX. An increase in the number of cells that are immunoreactive for proliferating cell nuclear antigen, a marker of dividing or recently mitotic cells, indicates that immature dentate gyrus cells divide following ADX. These results suggest that following ADX, mature cells born during the 1st postnatal week die, whereas immature cells divide.Keywords
This publication has 33 references indexed in Scilit:
- The Effects of Adrenal Steroids and Excitatory Input on Neuronal Birth and SurvivalaAnnals of the New York Academy of Sciences, 1994
- Adult neurogenesis is regulated by adrenal steroids in the dentate gyrusNeuroscience, 1994
- The cell cycle and cell deathCurrent Biology, 1993
- Bromodeoxyuridine immunohistochemical determination of the lengths of the cell cycle and the DNA-synthetic phase for an anatomically defined populationJournal of Neurocytology, 1989
- Neuronal death in the development of the vertebrate nervous systemTrends in Neurosciences, 1985
- Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA ReplicationScience, 1982
- Naturally occurring neuron death in the ganglion cell layer of the neonatal rat: morphology and evidence for regional correspondence with neuron death in superior colliculusDevelopmental Brain Research, 1981
- Neurogenesis in the Adult Rat: Electron Microscopic Analysis of Light RadioautographsScience, 1977
- An autoradiographic study of the time of origin and the pattern of granule cell migration in the dentate gyrus of the ratJournal of Comparative Neurology, 1975
- CELL DEATHS IN NORMAL VERTEBRATE ONTOGENYBiological Reviews, 1951