Mast Cell-Dependent Allergic Responses Are Inhibited by Ethanolic Extract of Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) Testa
- 26 January 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 58 (4), 2596-2601
- https://doi.org/10.1021/jf904356q
Abstract
Allergy is an immune dysfunction caused by degranulation from mast cells in the early phase and cytokine secretion in the late phase of the cell. The purpose of this study was to investigate the effects of adlay (Job’s tears, Coix lachryma-jobi L. var. ma-yuen Stapf) testa against β-hexosaminidase release as a marker of degranulation in rat basophilic leukemia (RBL)-2H3 cells. The ethyl acetate fraction from ethanolic extracts of adlay testa (ATE-EtOAc) exhibited potent inhibitory activity that suppressed degranulation from RBL-2H3 cells stimulated by 1 μM A23187. The 20%−80% EtOAc/Hex subfractions of ATE-EtOAc significantly inhibited histamine release with a IC50 of 75−100 μg/mL. In addition, the ATE-EtOAc subfractions suppressed interleukin (IL)-4, IL-6, and tumor necrosis factor-α secretion in RBL-2H3 cells, indicating that adlay testa were able to inhibit cytokine secretion. In order to explore the inhibitory mechanism of adlay testa in mast cell degranulation, we examined the activation of intracellular signaling molecules. Adlay testa inhibited the phosphorylation ERK expression. Furthermore, the two major active compounds, 4-hydroxyacetophenone and p-coumaric acid, were isolated from the ATE-EtOAc subfractions. These results suggest that ATE had an inhibitory effect on allergic response via the ERK signaling transduction in RBL-2H3 cells.Keywords
This publication has 20 references indexed in Scilit:
- Effect of Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) Testa and Its Phenolic Components on Cu2+-Treated Low-Density Lipoprotein (LDL) Oxidation and Lipopolysaccharide (LPS)-Induced Inflammation in RAW 264.7 MacrophagesJournal of Agricultural and Food Chemistry, 2009
- Role of mast cells in allergic and non-allergic immune responses: comparison of human and murine dataNature Reviews Immunology, 2007
- Anti-allergic substances from the rhizomes of Dioscorea membranaceaBioorganic & Medicinal Chemistry, 2006
- Modulating TNF-α signaling with natural productsDrug Discovery Today, 2006
- Enhancement of the release of inflammatory mediators by substance P in rat basophilic leukemia RBL-2H3 cellsJournal of Biomedical Science, 2006
- Integrated signalling pathways for mast-cell activationNature Reviews Immunology, 2006
- Anti-allergic principles from Thai zedoary: structural requirements of curcuminoids for inhibition of degranulation and effect on the release of TNF-α and IL-4 in RBL-2H3 cellsBioorganic & Medicinal Chemistry, 2004
- Suppression of Allergic Reactions by Dehulled Adlay in Association with the Balance of Th1/Th2 Cell ResponsesJournal of Agricultural and Food Chemistry, 2003
- Anti-Inflammatory Activity of Benzoxazinoids from Roots of Coix lachryma-jobi var. Ma-yuenJournal of Natural Products, 1988
- Isolation and Hypoglycemic Activity of Coixans A, B and C, Glycans ofCoix lachryma-jobivar.ma-yuenSeeds1Planta Medica, 1986