Improved flow cytometric detection of minimal residual disease in childhood acute lymphoblastic leukemia
- 16 August 2012
- journal article
- Published by Springer Science and Business Media LLC in Leukemia
- Vol. 27 (3), 635-641
- https://doi.org/10.1038/leu.2012.231
Abstract
Most current treatment protocols for acute lymphoblastic leukemia (ALL) include minimal residual disease (MRD) diagnostics, generally based on PCR analysis of rearranged antigen receptor genes. Although flow cytometry (FCM) can be used for MRD detection as well, discordant FCM and PCR results are obtained in 5-20% of samples. We evaluated whether 6-color FCM, including additional markers and new marker combinations, improved the results. Bone marrow samples were obtained from 363 ALL patients at day 15,33 and 78 and MRD was analyzed using 6-color (218 patients) or 4-color (145 patients) FCM in parallel to routine PCR-based MRD diagnostics. Compared with 4-color FCM, 6-color FCM significantly improved the concordance with PCR-based MRD data (88% versus 96%); particularly the specificity of the MRD analysis improved. However, PCR remained more sensitive at levelsThis publication has 51 references indexed in Scilit:
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