SecA protein is directly involved in protein secretion in Escherichia coli
- 2 January 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 242 (2), 431-434
- https://doi.org/10.1016/0014-5793(89)80516-2
Abstract
A high-expression plasmid for the secA gene was constructed. The SecA protein was then overproduced in E. coli and purified. The purified SecA stimulated the in vitro translocation of a model secretory protein into inverted membrane vesicles pretreated with 4 M urea. Membrane vesicles from a secAts mutant exhibited lower translocation activity, which was enhanced by SecA. These results indicate that SecA is directly involved in protein secretion across the cytoplasmic membraneThis publication has 13 references indexed in Scilit:
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