Determination of the protein composition of the occlusion-derived virus of Autographa californica nucleopolyhedrovirus
- 6 August 2003
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 100 (17), 9797-9802
- https://doi.org/10.1073/pnas.1733972100
Abstract
The occlusion derived form of baculovirus is specially adapted for primary infection of the host midgut epithelium. As such, the virion must contain the proteins essential for host range determination and initiation of infection. Because knowledge of virion composition is a prerequisite for functional investigation, this study used a combination of techniques to identify the proteins present within or associated with the occlusion-derived virus (ODV) virion. Thirty-one proteins, including proteins known to be essential for viral DNA replication, were identified with confidence. An additional 13 proteins were identified by using one of the three techniques. A comparison of gene conservation among the ODV proteins encoded in the 16 sequenced baculoviridae genomes is presented. With knowledge of the composition of ODV, it is now possible to target proteins and study their role(s) during primary infection.Keywords
This publication has 42 references indexed in Scilit:
- Data analysis—the Achilles heel of proteomicsNature Biotechnology, 2003
- Analysis of an Autographa californica Nucleopolyhedrovirus lef-11 Knockout: LEF-11 Is Essential for Viral DNA ReplicationJournal of Virology, 2002
- Kinetics of baculovirus replication and release using real‐time quantitative polymerase chain reactionBiotechnology & Bioengineering, 2002
- Furin Is Involved in Baculovirus Envelope Fusion Protein ActivationJournal of Virology, 2002
- Identification of BV/ODV-C42, an Autographa californica Nucleopolyhedrovirus orf101 -Encoded Structural Protein Detected in Infected-Cell Complexes with ODV-EC27 and p78/83Journal of Virology, 2001
- Impact of Recombinant Baculovirus Applications on Target Heliothines and Nontarget Predators in CottonBiological Control, 2000
- Identification of proteins in polyacrylamide gels by mass spectrometric peptide mapping combined with database searchJournal of Mass Spectrometry, 1994
- Analysis of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus trans-activators IE-1 and IE-2 using monoclonal antibodiesJournal of General Virology, 1993
- Immunocytochemical characterization of p24, a baculovirus capsid-associated proteinJournal of General Virology, 1993
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970