Stop-flow analysis of bile secretion

Abstract

Stop-flow analysis was performed on anesthetized rabbits after catheterization of common bile duct, the accessory lateral ducts and the cystic duct being tied. Occlusion of the duct lasted 15 min. BSP [bromsulfalein] injected at the 10th min. permitted the distinction in the bile channels between a proximal segment (bile canaliculi) and a distal segment (bile ducts). Glutamic oxalacetic transaminase was used as marker for H2O movements. In the distal segment, an electrolyte fluid isosmotic with primary bile is secreted into which an excess of K is added probably by goblet cells secretion. In the proximal segment, H2O is moved across hepatic cells by modifications of hydrostatic and/or osmotic pressure gradients. Bilirubin is secreted in the proximal segment even during bile duct occlusion and is not reabsorbed in the distal segment. Biliary alkaline phosphatase is secreted by hepatic cells of the proximal segment and by biliary cells of the distal segment as well as cleared from serum; its synthesis and biliary excretion is stimulated by bile duct obstruction.