Insights into SARS-CoV-2, the Coronavirus Underlying COVID-19: Recent Genomic Data and the Development of Reverse Genetics Systems
Open Access
- 1 October 2020
- journal article
- review article
- Published by Microbiology Society in Journal of General Virology
- Vol. 101 (10), 1021-1024
- https://doi.org/10.1099/jgv.0.001458
Abstract
The emergence and rapid worldwide spread of a novel pandemic of acute respiratory disease – eventually named coronavirus disease 2019 (COVID-19) by the World Health Organization (WHO) – across the human population has raised great concerns. It prompted a mobilization around the globe to study the underlying pathogen, a close relative of severe acute respiratory syndrome coronavirus (SARS-CoV) called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Numerous genome sequences of SARS-CoV-2 are now available and in-depth analyses are advancing. These will allow detailed characterization of sequence and protein functions, including comparative studies. Care should be taken when inferring function from sequence information alone, and reverse genetics systems can be used to unequivocally identify key features. For example, the molecular markers of virulence, host range and transmissibility of SARS-CoV-2 can be compared to those of related viruses in order to shed light on the biology of this emerging pathogen. Here, we summarize some recent insights from genomic studies and strategies for reverse genetics systems to generate recombinant viruses, which will be useful to investigate viral genome properties and evolution.Funding Information
- Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco
This publication has 46 references indexed in Scilit:
- Atlas of coronavirus replicase structureVirus Research, 2013
- Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirusProceedings of the National Academy of Sciences of the United States of America, 2013
- Human coronaviruses: Clinical features and phylogenetic analysisBiomedicine & Pharmacotherapy, 2013
- Restored PB1-F2 in the 2009 Pandemic H1N1 Influenza Virus Has Minimal Effects in SwineJournal of Virology, 2012
- Differential Contribution of PB1-F2 to the Virulence of Highly Pathogenic H5N1 Influenza A Virus in Mammalian and Avian SpeciesPLoS Pathogens, 2011
- Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAsMethods in molecular biology (Clifton, N.J.), 2008
- A Contemporary View of Coronavirus TranscriptionJournal of Virology, 2007
- Construction of a Severe Acute Respiratory Syndrome Coronavirus Infectious cDNA Clone and a Replicon To Study Coronavirus RNA SynthesisJournal of Virology, 2006
- Biochemical Aspects of Coronavirus Replication and Virus-Host InteractionAnnual Review of Microbiology, 2006
- Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirusProceedings of the National Academy of Sciences of the United States of America, 2003