In VivoGeneration of Cartilage from Periosteum
- 1 March 2005
- journal article
- research article
- Published by Mary Ann Liebert Inc in Tissue Engineering
- Vol. 11 (3-4), 369-377
- https://doi.org/10.1089/ten.2005.11.369
Abstract
Periosteum has chondrogenic and osteogenic potential and plays an important role in fracture healing. The purpose of this study was to evaluate the reactive tissue formed after damaging the periosteum. Damaging the periosteum may be a way to generate ectopic cartilage or bone, which may be useful for the repair of articular cartilage and bone defects. Periosteum was bilaterally dissected from the proximal medial tibia of New Zealand White rabbits. Reactive periosteal tissue was harvested 10, 20, and 40 days postsurgery and analyzed for expression of collagen types I, II, and X, aggrecan, osteopontin, and osteonectin (by reverse transcription-polymerase chain reaction) and collagen types I and II (by immunohistochemistry). Reactive tissue was present in 93% of cases. Histologically, this tissue consisted of hyaline cartilage at follow-up days 10 and 20. Expression of collagen type II and aggrecan was present at 10 and 20 days postsurgery. Highest expression was at 10 days. Expression of collagen type X increased up to 20 days. No significant changes in the mRNA expression of osteopontin or osteonectin were observed. Immunohistochemistry confirmed the presence of cartilage, which was positive for collagen types I and II at 10 days and only for collagen type II at 20 days. At 20 days postsurgery the onset of bone formation was also observed. At 40 days postsurgery, the reactive tissue had almost completely turned into bone. The quality and amount of cartilage formed 10 days postsurgery make this technique potentially useful to fill large cartilage and bone defects. Also, periosteal callus formation, providing possible useful information for tissueengineering techniques, can be studied with this model.Keywords
This publication has 28 references indexed in Scilit:
- In VivoMesenchymal Cell Recruitment by a Scaffold Loaded with Transforming Growth Factorβ1 and the Potential forin SituChondrogenesisTissue Engineering, 2002
- Assessment of specific mRNA levels in cartilage regions in a lapine model of osteoarthritisJournal of Orthopaedic Research, 2002
- Localization of chondrocyte precursors in periosteumOsteoarthritis and Cartilage, 2001
- Anatomical effects of periosteal elevationJournal of Orthopaedic Research, 2000
- Gene expression in menisci from the knees of skeletally immature and mature female rabbitsJournal of Orthopaedic Research, 1999
- Relationship of donor site to chondrogenic potential of periosteum in vitroJournal of Orthopaedic Research, 1994
- Collagen gene expression during chondrogenesis from chick periosteum‐derived cellsFEBS Letters, 1992
- In Vivo Osteochondrogenic Potential of Cultured Cells Derived From the PeriosteumPublished by Ovid Technologies (Wolters Kluwer Health) ,1990
- The effect of low oxygen concentration on growth, glycolysis, and sulfate incorporation by articular chondrocytes in monolayer cultureArthritis & Rheumatism, 1973