Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells
Open Access
- 24 June 2010
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 5 (6), e10964
- https://doi.org/10.1371/journal.pone.0010964
Abstract
During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is the basis for women's cycling and its dysfunction may be involved in the etiology of pathological disorders. Therefore, the human endometrial tissue must rely on a remarkable endometrial somatic stem cells (SSC) population. Here we explore the hypothesis that human endometrial side population (SP) cells correspond to somatic stem cells. We isolated, identified and characterized the SP corresponding to the stromal and epithelial compartments using endometrial SP genes signature, immunophenotyping and characteristic telomerase pattern. We analyzed the clonogenic activity of SP cells under hypoxic conditions and the differentiation capacity in vitro to adipogenic and osteogenic lineages. Finally, we demonstrated the functional capability of endometrial SP to develop human endometrium after subcutaneous injection in NOD-SCID mice. Briefly, SP cells of human endometrium from epithelial and stromal compartments display genotypic, phenotypic and functional features of SSC.Keywords
This publication has 57 references indexed in Scilit:
- Human circulating fibrocytes have the capacity to differentiate osteoblasts and chondrocytesThe International Journal of Biochemistry & Cell Biology, 2010
- Endometrial Cancer Side-Population Cells Show Prominent Migration and Have a Potential to Differentiate into the Mesenchymal Cell LineageThe American Journal of Pathology, 2010
- DNMT1 maintains progenitor function in self-renewing somatic tissueNature, 2010
- The longest telomeres: a general signature of adult stem cell compartmentsGenes & Development, 2008
- Side population in human uterine myometrium displays phenotypic and functional characteristics of myometrial stem cellsProceedings of the National Academy of Sciences of the United States of America, 2007
- Endometrial regenerative cells: A novel stem cell populationJournal of Translational Medicine, 2007
- Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statementCytotherapy, 2006
- Rank products: a simple, yet powerful, new method to detect differentially regulated genes in replicated microarray experimentsFEBS Letters, 2004
- Reprogramming in Inter-Species Embryonal Carcinoma–Somatic Cell Hybrids Induces Expression of Pluripotency and Differentiation MarkersCloning and Stem Cells, 2003
- Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo.The Journal of Experimental Medicine, 1996