Subchronic inhalation toxicity of gold nanoparticles
Open Access
- 14 May 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in Particle and Fibre Toxicology
- Vol. 8 (1), 16
- https://doi.org/10.1186/1743-8977-8-16
Abstract
Gold nanoparticles are widely used in consumer products, including cosmetics, food packaging, beverages, toothpaste, automobiles, and lubricants. With this increase in consumer products containing gold nanoparticles, the potential for worker exposure to gold nanoparticles will also increase. Only a few studies have produced data on the in vivo toxicology of gold nanoparticles, meaning that the absorption, distribution, metabolism, and excretion (ADME) of gold nanoparticles remain unclear. The toxicity of gold nanoparticles was studied in Sprague Dawley rats by inhalation. Seven-week-old rats, weighing approximately 200 g (males) and 145 g (females), were divided into 4 groups (10 rats in each group): fresh-air control, low-dose (2.36 × 104 particle/cm3, 0.04 μg/m3), middle-dose (2.36 × 105 particle/cm3, 0.38 μg/m3), and high-dose (1.85 × 106 particle/cm3, 20.02 μg/m3). The animals were exposed to gold nanoparticles (average diameter 4-5 nm) for 6 hours/day, 5 days/week, for 90-days in a whole-body inhalation chamber. In addition to mortality and clinical observations, body weight, food consumption, and lung function were recorded weekly. At the end of the study, the rats were subjected to a full necropsy, blood samples were collected for hematology and clinical chemistry tests, and organ weights were measured. Cellular differential counts and cytotoxicity measurements, such as albumin, lactate dehydrogenase (LDH), and total protein were also monitored in a cellular bronchoalveolar lavage (BAL) fluid. Among lung function test measurements, tidal volume and minute volume showed a tendency to decrease comparing control and dose groups during the 90-days of exposure. Although no statistically significant differences were found in cellular differential counts, histopathologic examination showed minimal alveoli, an inflammatory infiltrate with a mixed cell type, and increased macrophages in the high-dose rats. Tissue distribution of gold nanoparticles showed a dose-dependent accumulation of gold in only lungs and kidneys with a gender-related difference in gold nanoparticles content in kidneys. Lungs were the only organ in which there were dose-related changes in both male and female rats. Changes observed in lung histopathology and function in high-dose animals indicate that the highest concentration (20 μg/m3) is a LOAEL and the middle concentration (0.38 μg/m3) is a NOAEL for this study.Keywords
This publication has 35 references indexed in Scilit:
- Impact of agglomeration state of nano- and submicron sized gold particles on pulmonary inflammationParticle and Fibre Toxicology, 2010
- Effects of nanomaterial physicochemical properties on in vivo toxicityAdvanced Drug Delivery Reviews, 2009
- Particle deposition in human respiratory system: Deposition of concentrated hygroscopic aerosolsInhalation Toxicology, 2009
- Subchronic Inhalation Toxicity of Silver NanoparticlesToxicological Sciences, 2008
- Long-Term Stability Characteristics of Metal Nanoparticle Generator Using Small Ceramic Heater for Inhalation Toxicity StudiesInhalation Toxicology, 2007
- Ultrafine Particle–Lung Interactions: Does Size Matter?Journal of Aerosol Medicine, 2006
- Gold, the Noble Metal and the Paradoxes of its ToxicologyBiologicals, 1998
- Pneumotoxicity and Pulmonary Clearance of Different Welding Fumes after Intratracheal Instillation in the RatToxicology and Applied Pharmacology, 1996
- DISTINCTIVE PROFILE OF ALVEOLAR MACROPHAGEDERIVED CYTOKINE RELEASE INDUCED BY FIBROGENIC AND NONFIBROGENIC MINERAL DUSTSJournal of Toxicology and Environmental Health, 1996
- A case of contact hypersensitivity to metallic goldArchives of Dermatology, 1969