Identification of Human T-lymphotropic Virus Type I (HTLV-I) Subtypes Using Restrited Fragment Length Polymorphism in a Cohort of Asymptomatic Carriers and Patients with HTLV-I-associated Myelopathy/tropical Spastic Paraparesis from São Paulo, Brazil
Open Access
- 1 April 2002
- journal article
- Published by FapUNIFESP (SciELO) in Memórias do Instituto Oswaldo Cruz
- Vol. 97 (3), 329-333
- https://doi.org/10.1590/s0074-02762002000300009
Abstract
Although human T-lymphotropic virus type I (HTLV-I) exhibits high genetic stability, as compared to other RNA viruses and particularly to human immunodeficiency virus (HIV), genotypic subtypes of this human retrovirus have been characterized in isolates from diverse geographical areas. These are currently believed not to be associated with different pathogenetic outcomes of infection. The present study aimed at characterizing genotypic subtypes of viral isolates from 70 HTLV-I-infected individuals from São Paulo, Brazil, including 42 asymptomatic carriers and 28 patients with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), using restricted fragment length polymorphism (RFLP) analysis of long terminal repeat (LTR) HTLV-I proviral DNA sequences. Peripheral blood mononuclear cell lysates were amplified by nested polymerase chain reaction (PCR) and amplicons submitted to enzymatic digestion using a panel of endonucleases. Among HTLV-I asymptomatic carriers, viral cosmopolitan subtypes A, B, C and E were identified in 73.8%, 7.1%, 7.1% and 12% of tested samples, respectively, whereas among HAM/TSP patients, cosmopolitan A (89.3%), cosmopolitan C (7.1%) and cosmopolitan E (3.6%) subtypes were detected. HTLV-I subtypes were not statistically significant associated with patients' clinical status. We also conclude that RFLP analysis is a suitable tool for descriptive studies on the molecular epidemiology of HTLV-I infections in our environmentKeywords
This publication has 28 references indexed in Scilit:
- HTLV-I-Associated Myelopathy/Tropical Spastic Paraparesis in Brazil: A Nationwide SurveyJAIDS Journal of Acquired Immune Deficiency Syndromes, 1998
- HTLV-I in Northeast Brazil: Differences for Male and Female Injecting Drug UsersJAIDS Journal of Acquired Immune Deficiency Syndromes, 1998
- Prevalence of HTLV-I and HTLV-II infections among HIV-1-infected asymptomatic individuals in São Paulo, BrazilRevista do Instituto de Medicina Tropical de São Paulo, 1997
- Distribution of human T‐lymphotropic virus type I among blood donors: a nationwide Brazilian studyTransfusion, 1997
- HTLV infection in a group of prostitutes and their male sexual clients in Brazil: seroprevalence and risk factorsTransactions of the Royal Society of Tropical Medicine and Hygiene, 1996
- Human T-lymphotropic virus type I and type II infections and correlation with risk factors in blood donors from São Paulo, BrazilTransfusion, 1995
- Geographical Subtypes Demonstrated by RFLP Following PCR in the LTR Region of HTLV-IAIDS Research and Human Retroviruses, 1992
- The prevalence of antibody to HTLV‐I/II in United States plasma donors and in United States and French hemophiliacsTransfusion, 1990
- HTLV-I associated myelopathy in Brazil: a preliminary reportArquivos de Neuro-Psiquiatria, 1989
- HIV-1, HIV-2, and HTLV-I Infection in High-Risk Groups in BrazilNew England Journal of Medicine, 1989