Viral Replicase Gene Products Suffice for Coronavirus Discontinuous Transcription
Open Access
- 15 July 2001
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 75 (14), 6676-6681
- https://doi.org/10.1128/jvi.75.14.6676-6681.2001
Abstract
We have used vaccinia virus as a vector to clone a 22.5-kbp cDNA that represents the 5′ and 3′ ends of the human coronavirus 229E (HCoV 229E) genome, the HCoV 229E replicase gene, and a single reporter gene (coding for green fluorescent protein [GFP]) located downstream of a regulatory element for coronavirus mRNA transcription. When RNA transcribed from this cDNA was transfected into BHK-21 cells, a small percentage of cells displayed strong fluorescence. A region of the mRNA encoding GFP was amplified by PCR and shown to have the unique mRNA leader-body junction indicative of coronavirus-mediated transcription. These data show that the coronavirus replicase gene products suffice for discontinuous subgenomic mRNA transcription.Keywords
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