Identification of Clinically Relevant Enterococcus Species by Direct Sequencing of groES and Spacer Region
Open Access
- 1 January 2005
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 43 (1), 235-241
- https://doi.org/10.1128/jcm.43.1.235-241.2005
Abstract
We determined the groESL sequences ( groES , groEL , and the intergenic spacer) of 10 clinically relevant Enterococcus species and evaluated the feasibility of identifying Enterococcus species on the basis of these sequences. Seven common clinical Enterococcus species, E. faecalis , E. faecium , E. casseliflavus , E. gallinarum , E. avium , E. raffinosus , and E. hirae , and three less common Enterococcus species, E. cecorum , E. durans , and E. mundtii , were examined in this study. We found that the groES genes of these enterococcal species are identical in length (285 nucleotides) and contain an unusual putative start codon, GTG. The lengths and sequences of the intergenic regions (spacers between the groES and groEL genes) are quite variable (17 to 57 bp in length) among Enterococcus species but are conserved in strains within each species, with only a few exceptions. Considerable variation of groES or groEL sequences was also observed. The evolutionary trees of groES or groEL sequences revealed similarities among Enterococcus species. However, the overall intraspecies variation of groES was less than that of groEL . The high interspecies variation and low intraspecies variation indicate that the groES and spacer sequences are more useful than groEL for identification of clinically relevant Enterococcus species. The sequences of these two genetic traits, groES and spacer, can be determined by a single PCR and direct sequencing and may provide important information for the differentiation of closely related species of Enterococcus .Keywords
This publication has 24 references indexed in Scilit:
- groESL Sequence Determination, Phylogenetic Analysis, and Species Differentiation for Viridans Group StreptococciJournal of Clinical Microbiology, 2002
- Determination of Enterococcus faecalis groESL Full-Length Sequence and Application for Species IdentificationJournal of Clinical Microbiology, 2001
- Vertebral Osteomyelitis Caused by Enterococcus raffinosusJournal of Clinical Microbiology, 2001
- Routine Molecular Identification of Enterococci by Gene-Specific PCR and 16S Ribosomal DNA SequencingJournal of Clinical Microbiology, 2001
- Relationships between Enterococcal Virulence and Antimicrobial ResistanceClinical Microbiology Reviews, 2000
- Emergence of Vancomycin-Resistant Enterococci at a University Hospital in Taiwan: Persistence of Multiple Species and Multiple ClonesInfection Control & Hospital Epidemiology, 1999
- Evaluation of intraspecies genetic variation within the 60 kDa heat-shock protein gene (groEL) of Bartonella speciesInternational Journal of Systematic and Evolutionary Microbiology, 1999
- Cloning, sequencing and molecular analysis of the Campylobacter jejuni groESL bicistronic operonMicrobiology, 1999
- Division of the genus Enterococcus into species groups using PCR-based molecular typing methodsMicrobiology, 1998
- Folding in vivo of bacterial cytoplasmic proteins: Role of GroELCell, 1993