Crystal structure of the kainate receptor GluR5 ligand‐binding core in complex with (S)‐glutamate
Open Access
- 19 January 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (5), 1154-1160
- https://doi.org/10.1016/j.febslet.2005.01.012
Abstract
The X‐ray structure of the ligand‐binding core of the kainate receptor GluR5 (GluR5‐S1S2) in complex with (S)‐glutamate was determined to 1.95 Å resolution. The overall GluR5‐S1S2 structure comprises two domains and is similar to the related AMPA receptor GluR2‐S1S2J. (S)‐glutamate binds as in GluR2‐S1S2J. Distinct features are observed for Ser741, which stabilizes a highly coordinated network of water molecules and forms an interdomain bridge. The GluR5 complex exhibits a high degree of domain closure (26°) relative to apo GluR2‐S1S2J. In addition, GluR5‐S1S2 forms a novel dimer interface with a different arrangement of the two protomers compared to GluR2‐S1S2J.Keywords
This publication has 33 references indexed in Scilit:
- The Three-dimensional Structure of an Ionotropic Glutamate Receptor Reveals a Dimer-of-dimers AssemblyJournal of Molecular Biology, 2004
- Probing the Function, Conformational Plasticity, and Dimer−Dimer Contacts of the GluR2 Ligand-Binding Core: Studies of 5-Substituted Willardiines and GluR2 S1S2 in the Crystal,Biochemistry, 2003
- Glutamate receptor function in learning and memoryBehavioural Brain Research, 2003
- Competitive Antagonism of AMPA Receptors by Ligands of Different Classes: Crystal Structure of ATPO Bound to the GluR2 Ligand-Binding Core, in Comparison with DNQXJournal of Medicinal Chemistry, 2002
- Mechanism of glutamate receptor desensitizationNature, 2002
- Refinement of Macromolecular Structures by the Maximum-Likelihood MethodActa Crystallographica Section D-Biological Crystallography, 1997
- [20] Processing of X-ray diffraction data collected in oscillation modeMethods in Enzymology, 1997
- VMD: Visual molecular dynamicsJournal of Molecular Graphics, 1996
- The CCP4 suite: programs for protein crystallographyActa Crystallographica Section D-Biological Crystallography, 1994
- MOLSCRIPT: a program to produce both detailed and schematic plots of protein structuresJournal of Applied Crystallography, 1991