Acetyicholinesterase secretion–a parameter for the interpretation of in vitro anthelmintic screens
- 1 February 1986
- journal article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 92 (2), 425-430
- https://doi.org/10.1017/s0031182000064180
Abstract
Interpretation of anthelmintic activity using in vitro screens has, until now, relied on the detection of drug-induced effects on nematode development, viability and motility. A novel biochemical parameter dependent upon the spectrophotometric assay of acetyicholinesterase (AChE), an enzyme secreted in large quantities by certain trichostrongylid nematodes, has been developed to replace these often subjective indices of activity. Using Nippostrongylus brasiliensis, a worm frequently employed for primary screening, the secretion of this enzyme in the presence or absence of a large number of drugs in vitro was determined. During a 4-day incubation period in a complex undefined medium without serum, AChE was secreted by normal 4th larval and immature adult stages of the worm in a linear fashion. All modern broad-spectrum veterinary anthelmintics, regardless of their mode of action, dramatically reduced the amount of enzyme secreted. Correlation between the biochemical and observa tional parameters was excellent and the selectivity of the assay when based solely on enzyme secretion was not lost. Other advantages were that the time required for the activity of certain slow-acting compounds to be detected was reduced from 7 to 4 days and that close microscopical examination of the worms was not necessary.Keywords
This publication has 7 references indexed in Scilit:
- Nematospiroides dubius: response of the late fourth-stage larva to anthelmintics in vitroZeitschrift für Parasitenkunde, 1984
- An in vitro screen for anthelmintics employing Nippostrongylus brasiliensis in a defined mediumVeterinary Parasitology, 1982
- In vitro Screening Tests for AnthelminticsPublished by Springer Science and Business Media LLC ,1982
- In vitro maintenance of nematode parasites assayed by acetylcholinesterase and allergen secretionExperimental Parasitology, 1975
- Acetylcholinesterase secretion by parasitic nematodes—I. Evidence for secretion of the enzyme by a number of speciesInternational Journal for Parasitology, 1973
- Cholinesterase activity in Haemonchus contortus and its inhibition by organophosphorus anthelminticsBiochemical Pharmacology, 1963
- A new and rapid colorimetric determination of acetylcholinesterase activityBiochemical Pharmacology, 1961