Dibutyl phthalate biodegradation by the white rot fungus, Polyporus brumalis
- 12 January 2007
- journal article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 97 (6), 1516-1522
- https://doi.org/10.1002/bit.21333
Abstract
In this study, white rot fungus, Polyporus brumalis, was applied to degrade dibutyl phthalate (DBP), a major environmental pollutant. The degradation potential and resulting products were evaluated with HPLC and GC/MS. As DBP concentration increased to 250, 750, and 1,250 µM, the mycelial growth of P. brumalis was inhibited. However, growth was still observed in the 1,250 µM concentration. DBP was nearly eliminated from culture medium of P. brumalis within 12 days, with 50% of DBP adsorbed by the mycelium. Diethyl phthalate (DEP) and monobutyl phthalate (MBP) were detected as intermediate degradation products of DBP. In culture medium, the concentration of DEP was higher than that of MBP during the incubation period. After 12–15 days, the concentrations of both decreased rapidly in the culture medium. The primary final degradation product of DBP in culture medium was phthalic acid anhydride, as well as trace amounts of aromatic compounds, such as α‐hydroxyphenylacetic acid, benzyl alcohol, and O‐hydroxyphenylacetic acid. According to these results, the degradation of DBP in culture medium by the white rot fungus, P. brumalis, may be completed through two pathways—transesterification and de‐esterification—which successively combine into an intracellular degradation pathway. Biotechnol. Bioeng. 2007; 97: 1516–1522.This publication has 16 references indexed in Scilit:
- Biodegradation of Phthalic Acid Esters by Bakery Yeast Saccharomyces cerevisiaeBulletin of Environmental Contamination and Toxicology, 2003
- Biodegradation Characteristics of Monochlorophenols by Wood Rot FungiKorean Journal of Environmental Agriculture, 2002
- Enhanced Degradation of an Endocrine-Disrupting Chemical, Butyl Benzyl Phthalate, byFusarium oxysporumf. sp.pisiCutinaseApplied and Environmental Microbiology, 2002
- Biodegradation of diethyl phthalate in soil by a novel pathwayFEMS Microbiology Letters, 2000
- Fungal delignification and biomechanical pulping of woodAdvances in Biochemical Engineering/Biotechnology, 1997
- THE β-KETOADIPATE PATHWAY AND THE BIOLOGY OF SELF-IDENTITYAnnual Review of Microbiology, 1996
- A variety of environmentally persistent chemicals, including some phthalate plasticizers, are weakly estrogenic.Environmental Health Perspectives, 1995
- H2O2 Recycling during Oxidation of the Arylglycerol .beta.-Aryl Ether Lignin Structure by Lignin Peroxidase and Glyoxal OxidaseBiochemistry, 1994
- Ring fission of anthracene by a eukaryote.Proceedings of the National Academy of Sciences of the United States of America, 1991
- Production of multiple ligninases by Phanerochaete chrysosporium: effect of selected growth conditions and use of a mutant strainEnzyme and Microbial Technology, 1986