A ChemiluminesClent Precolumn Labelling Reagent for High-Performance Liquid Chromatography of Amino Acids
- 1 January 1986
- journal article
- analytical biochemistry-and-clinical-analysis
- Published by Taylor & Francis Ltd in Analytical Letters
- Vol. 19 (23-24), 2277-2283
- https://doi.org/10.1080/00032718608064553
Abstract
A chemiluminescent tag for precolumn derivatization of amino acids has been developed. The tag, 4-isocyanatophthalhydrazide, couples with 17 amino acids tested, including proline and hydroxyproline, in less than ten minutes. Twelve derivatized amino acids have been separated and detected with an average detection limit of 10 femptomoles per 20 μL injection.Keywords
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