Defective RNA Replication and Late Gene Expression in Temperature-Sensitive Influenza Viruses Expressing Deleted Forms of the NS1 Protein
- 15 April 2004
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 78 (8), 3880-3888
- https://doi.org/10.1128/jvi.78.8.3880-3888.2004
Abstract
Influenza A virus mutants expressing C-terminally deleted forms of the NS1 protein (NS1-81 and NS1-110) were generated by plasmid rescue. These viruses were temperature sensitive and showed a small plaque size at the permissive temperature. The accumulation of virion RNA in mutant virus-infected cells was reduced at the restrictive temperature, while the accumulation of cRNA or mRNA was not affected, indicating that the NS1 protein is involved in the control of transcription versus replication processes in the infection. The synthesis and accumulation of late virus proteins were reduced in NS1-81 mutant-infected cells at the permissive temperature and were essentially abolished for both viruses at the restrictive temperature, while synthesis and accumulation of nucleoprotein (NP) were unaffected. Probably as a consequence, the nucleocytoplasmic export of virus NP was strongly inhibited at the restrictive temperature. These results indicate that the NS1 protein is essential for nuclear and cytoplasmic steps during the virus cycle.This publication has 70 references indexed in Scilit:
- NS1 Protein of Influenza A Virus Down-Regulates ApoptosisJournal of Virology, 2002
- Effects of Influenza A Virus NS1 Protein on Protein Expression: the NS1 Protein Enhances Translation and Is Not Required for Shutoff of Host Protein SynthesisJournal of Virology, 2002
- Multiple Levels of Posttranscriptional Regulation of Influenza Virus Gene ExpressionSeminars in Virology, 1998
- Amino acid replacements leading to temperature-sensitive defects of the NS1 protein of influenza A virusArchiv für die gesamte Virusforschung, 1995
- Influenza virus naked RNA can be expressed upon transfection into cells co-expressing the three subunits of the polymerase and the nucleoprotein from simian virus 40 recombinant virusesJournal of General Virology, 1993
- Nuclear transport of influenza virus polymerase PA proteinVirus Research, 1992
- Degradation of cellular mRNA during influenza virus infection: its possible role in protein synthesis shutoffJournal of General Virology, 1992
- Specific binding of influenza A virus NS1 protein to the virus minus-sense RNA in vitroJournal of General Virology, 1992
- Nuclear transport of influenza virus ribonucleoproteins: The viral matrix protein (M1) promotes export and inhibits importCell, 1991
- Influenza virus, an RNA virus, synthesizes its messenger RNA in the nucleus of infected cellsCell, 1981