Not all butterfly eyes are created equal: Rhodopsin absorption spectra, molecular identification, and localization of ultraviolet‐, blue‐, and green‐sensitive rhodopsin‐encoding mRNAs in the retina of Vanessa cardui
- 26 February 2003
- journal article
- research article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 458 (4), 334-349
- https://doi.org/10.1002/cne.10582
Abstract
Surveys of spectral sensitivities, visual pigment spectra, and opsin gene sequences have indicated that all butterfly eyes contain ultraviolet‐, blue‐, and green‐sensitive rhodopsins. Some species also contain a fourth or fifth type, related in amino acid sequence to green‐sensitive insect rhodopsins, but red shifted in absorbance. By combining electron microscopy, epi‐microspectrophotometry, and polymerase chain reaction cloning, we found that the compound eye of Vanessa cardui has the typical ultrastructural features of the butterfly retina but contains only the three common insect rhodopsins. We estimated lambda‐max values and relative densities of the rhodopsins in the Vanessa retina (0.72, P530; 0.12, P470; and 0.15, P360) from microspectrophotometric measurements and calculations based on a computational model of reflectance spectra. We isolated three opsin‐encoding cDNA fragments that were identified with P530, P470, and P360 by homology to the well‐characterized insect rhodopsin families. The retinal mosaic was mapped by opsin mRNA in situ hybridization and found to contain three kinds of ommatidia with respect to their patterns of short wavelength rhodopsin expression. In some ommatidia, P360 or P470 was expressed in R1 and R2 opposed receptor cells; in others, one cell expressed P360, whereas its complement expressed P470. P530 was expressed in the other seven cells of all ommatidia. P470‐expressing cells were abundant in the ventral retina but nearly absent dorsally. Our results indicated that there are major differences between the color vision systems of nymphalid and papilionid butterflies: the nymphalid Vanessa has a simpler, trichromatic, system than do the tetrachromatic papilionids that have been studied. J. Comp. Neurol. 458:334–349, 2003.Keywords
This publication has 51 references indexed in Scilit:
- Ommatidial heterogeneity in the compound eye of the male small white butterfly, Pieris rapae crucivoraCell and tissue research, 2002
- Molecular evolution of a long wavelength-sensitive opsin in mimetic Heliconius butterflies (Lepidoptera: Nymphalidae)Biological Journal of the Linnean Society, 2001
- THEEVOLUTION OFCOLORVISION ININSECTSAnnual Review of Entomology, 2001
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- The role of retinal photoisomerase in the visual cycle of the honeybee.The Journal of general physiology, 1991
- Properties of the visual pigments of the moth Manduca sexta and the effects of two detergents, digitonin and CHAPSVision Research, 1985
- Bleaching of Rhabdoms in Eyes of Intact ButterfliesScience, 1983
- Dark-processes following photoconversion of butterfly rhodopsinsEuropean Biophysics Journal, 1983
- Red-Absorbing Visual Pigment of ButterfliesScience, 1979
- Butterfly glowJournal of Ultrastructure Research, 1968