The Protection of Selenium against Cadmium-Induced Cytotoxicity via the Heat Shock Protein Pathway in Chicken Splenic Lymphocytes
Open Access
- 7 December 2012
- Vol. 17 (12), 14565-14572
- https://doi.org/10.3390/molecules171214565
Abstract
Cadmium (Cd) is a heavy metal that poses a hazard to animal health due to its toxicity. Selenium (Se) is an important nutritional trace element. However, the potential protective effects of Se against Cd-induced toxicity remain to be elucidated. To investigate the cytotoxicity of Cd on bird immunocytes in vitro and the protective effects of Se against exposure to Cd, chicken splenic lymphocytes received Cd (10−6 mol/L), Se (10−7 mol/L), and the mixture of 10−7 mol/L Se and 10−6 mol/L Cd and were incubated for 12 h, 24 h, 36 h, 48 h, respectively. The transcription of heat shock protein (HSP) 27, HSP40, HSP60, HSP70 and HSP90 mRNA was tested by fluorescence quantitative PCR. The results showed that the mRNA expression of HSPs exposed to 10−6 mol/L Cd showed a sustained decrease at 12–48 h exposure. A statistically significant increase in the mRNA expression of HSPs in the case of Se group was observed, as compared to the control group of chicken splenic lymphocytes. Concomitantly, treatment of chicken splenic lymphocytes with Se in combination with Cd enhanced the mRNA expression of HSPs which were reduced by Cd treatment. This indicated that the protective effect of Se against the toxicity of Cd might, at least partially, be attributed to stimulation of the level of HSPs.This publication has 38 references indexed in Scilit:
- Prenatal cadmium exposure alters postnatal immune cell development and functionToxicology and Applied Pharmacology, 2012
- Testicular toxicity induced by dietary cadmium in cocks and ameliorative effect by seleniumBioMetals, 2010
- The effect of dietary selenium supplementation on cadmium absorption and retention in suckling ratsBioMetals, 2009
- Effects of selenomethionine supplementation on selenium status and thyroid hormone concentrations in healthy adultsThe American Journal of Clinical Nutrition, 2009
- Cadmium-induced Activation of Stress Signaling Pathways, Disruption of Ubiquitin-dependent Protein Degradation and Apoptosis in Primary Rat Sertoli Cell-Gonocyte CoculturesToxicological Sciences, 2008
- Influence of cadmium on murine thymocytes: Potentiation of apoptosis and oxidative stressToxicology Letters, 2006
- Phenotypic anchoring of arsenic and cadmium toxicity in three hepatic-related cell systems reveals compound- and cell-specific selective up-regulation of stress protein expression: Implications for fingerprint profiling of cytotoxicityChemico-Biological Interactions, 2006
- Oxidative stress and apoptotic changes in murine splenocytes exposed to cadmiumToxicology, 2006
- Is there a role of glutathione peroxidases in signaling and differentiation?BioFactors, 2003
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001