Effects of Nelumbo nucifera Rhizome Extract on Cell Proliferation and Neuroblast Differentiation in the Hippocampal Dentate Gyrus in a Scopolamine‐induced Amnesia Animal Model
- 17 November 2010
- journal article
- research article
- Published by Wiley in Phytotherapy Research
- Vol. 25 (6), 809-815
- https://doi.org/10.1002/ptr.3337
Abstract
A large aquatic herb, Nelumbo nucifera Gaertn, has psychopharmacological effects similar to minor tranquillizers and antistress agents. This study examined the effects of Nelumbo nucifera rhizome extracts (NRE) on cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus (DG) of a rat model of scopolamine‐induced amnesia. Immunohistochemical markers included Ki67, an endogenous marker for active cell cycle, and doublecortin (DCX), a marker for immature neurons and migratory neuroblasts. Scopolamine was administered for 28 days via an ALzet minipump (44 mg/mL delivered at 2.5 µL/h). NRE was administered by gavage, 1 g/kg per day for 28 days. The administration of scopolamine significantly reduced the number of Ki67‐ and DCX‐immunoreactive cells in the DG, whereas scopolamine did not induce any significant changes in mature neurons in the DG. The administration of NRE significantly ameliorated the scopolamine‐induced reduction of Ki67‐ and DCX‐immunoreactive cells in the DG. In addition, the administration of NRE significantly restored the scopolamine‐induced reduction of brain‐derived neurotrophic factor in DG homogenates. These results suggest that NRE can ameliorate the scopolamine‐induced reductions of cell proliferation, neuroblast differentiation and BDNF levels. Copyright © 2010 John Wiley & Sons, Ltd.Keywords
This publication has 25 references indexed in Scilit:
- New neurons and new memories: how does adult hippocampal neurogenesis affect learning and memory?Nature Reviews Neuroscience, 2010
- Knockdown of brain-derived neurotrophic factor in specific brain sites precipitates behaviors associated with depression and reduces neurogenesisMolecular Psychiatry, 2009
- Novel effects of Nelumbo nucifera rhizome extract on memory and neurogenesis in the dentate gyrus of the rat hippocampusNeuroscience Letters, 2008
- Donepezil, an acetylcholinesterase inhibitor, enhances adult hippocampal neurogenesisChemico-Biological Interactions, 2008
- K252a, an inhibitor of Trk, disturbs pathfinding of hippocampal mossy fibersNeuroReport, 2006
- Increased neurogenesis and the ectopic granule cells after intrahippocampal BDNF infusion in adult ratsExperimental Neurology, 2005
- Doublecortin expression levels in adult brain reflect neurogenesisEuropean Journal of Neuroscience, 2005
- Decreased neurogenesis after cholinergic forebrain lesion in the adult ratJournal of Neuroscience Research, 2004
- Intraventricular Infusion of TrkB-Fc Fusion Protein Promotes Ischemia-Induced Neurogenesis in Adult Rat Dentate GyrusStroke, 2003
- Mammalian Neural Stem CellsScience, 2000