Estrogen oversees the maintenance of the female genetic program in terminally differentiated gonochorists
Open Access
- 7 October 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 3 (1), 2862
- https://doi.org/10.1038/srep02862
Abstract
The sexual plasticity of the gonads is not retained after the completion of sex differentiation in vertebrates, except in some hermaphroditic species. Here, we report that the depletion of estradiol-17β (E2) by aromatase inhibitors (AI) for up to six months resulted in a functional female-to-male sex reversal in sexually-mature adults of two gonochoristic fish species, Nile tilapia and medaka. The sex-reversed fish showed a typical male pattern of E2 and androgen levels, secondary sexual characteristics, and male-like sex behavior, producing fertile sperm. Conversely, co-treatment of E2 inhibited AI-induced sex reversal. In situ hybridization of medaka gonads during AI-induced sex reversal indicated that cysts on the dorsal side of the adult ovaries are the origin of germ cells and Sertoli cells in the newly formed testicular tissue. Gonochoristic fish maintain their sexual plasticity until adulthood and E2 plays a critical role in maintaining the female phenotype.Keywords
This publication has 33 references indexed in Scilit:
- Generation of functional eggs and sperm from cryopreserved whole testesProceedings of the National Academy of Sciences of the United States of America, 2013
- DMRT1 prevents female reprogramming in the postnatal mammalian testisNature, 2011
- Somatic Sex Reprogramming of Adult Ovaries to Testes by FOXL2 AblationCell, 2009
- Sex determination: insights from the chickenBioEssays, 2004
- A duplicated copy of DMRT1 in the sex-determining region of the Y chromosome of the medaka, Oryzias latipesProceedings of the National Academy of Sciences of the United States of America, 2002
- Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influencesAquaculture, 2002
- DMY is a Y-specific DM-domain gene required for male development in the medaka fishNature, 2002
- Aromatase Enzyme Activity and Sex Determination in ChickensScience, 1992
- Expression of a candidate sex-determining gene during mouse testis differentiationNature, 1990
- A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motifNature, 1990