Humidity-Dependent Decay of Viruses, but Not Bacteria, in Aerosols and Droplets Follows Disinfection Kinetics
Top Cited Papers
- 30 December 2019
- journal article
- research article
- Published by American Chemical Society (ACS) in Environmental Science & Technology
- Vol. 54 (2), 1024-1032
- https://doi.org/10.1021/acs.est.9b04959
Abstract
The transmission of some infectious diseases requires that pathogens can survive (i.e., remain infectious) in the environment, outside the host. Relative humidity (RH) is known to affect the survival of some microorganisms in the environment; however, the mechanism underlying the relationship has not been explained, particularly for viruses. We investigated the effects of RH on the viability of bacteria and viruses in both suspended aerosols and stationary droplets using traditional culture-based approaches. Results showed that viability of bacteria generally decreased with decreasing RH. Viruses survived well at RHs lower than 33% and at 100%, whereas their viability was reduced at intermediate RHs. We then explored the evaporation rate of droplets consisting of culture media and the resulting changes in solute concentrations over time; as water evaporates from the droplets, solutes such as sodium chloride in the media become more concentrated. Based on the results, we suggest that inactivation of bacteria is influenced by osmotic pressure resulting from elevated concentration of salts as droplets evaporate. We propose that the inactivation of viruses is governed by the cumulative dose of solutes, or the product of concentration and time, as in disinfection kinetics. These findings emphasize that evaporation kinetics play a role in modulating the survival of microorganisms in droplets.Keywords
Funding Information
- NIH Office of the Director (1-DP2-A1112243)
- Division of Electrical, Communications and Cyber Systems (ECCS-1542100)
- Division of Chemical, Bioengineering, Environmental, and Transport Systems (CBET-1438103)
This publication has 70 references indexed in Scilit:
- Comparing the mechanism of water condensation and evaporation in glassy aerosolProceedings of the National Academy of Sciences of the United States of America, 2012
- Impact of Internal RNA on Aggregation and Electrokinetics of Viruses: Comparison between MS2 Phage and Corresponding Virus-Like ParticlesApplied and Environmental Microbiology, 2011
- Global Influenza Seasonality: Reconciling Patterns across Temperate and Tropical RegionsEnvironmental Health Perspectives, 2011
- Evaporation kinetics and phase of laboratory and ambient secondary organic aerosolProceedings of the National Academy of Sciences of the United States of America, 2011
- The effect of environmental parameters on the survival of airborne infectious agentsJournal of The Royal Society Interface, 2009
- Absolute humidity modulates influenza survival, transmission, and seasonalityProceedings of the National Academy of Sciences of the United States of America, 2009
- Methods for Sampling of Airborne VirusesMicrobiology and Molecular Biology Reviews, 2008
- Seasonal infectious disease epidemiologyProceedings Of The Royal Society B-Biological Sciences, 2006
- Seasonality and the dynamics of infectious diseasesEcology Letters, 2006
- Seasonal Patterns of Infectious DiseasesPLoS Medicine, 2005