Heterophile Hanganutziu-Deicher Antigen in Ganglioside Fractions of Human Melanoma Tissues

Abstract

Gangliosides were purified from melanoma tissue extracts obtained from 5 patients. GM3 and GD3 were identified as the major components in ganglioside fractions of the melanoma tissues. Following thin-layer chromatography, enzyme immunostaining with Hanganutziu-Deicher (H-D) antigen-specific chicken antisera demonstrated the presence of NeuGc-neolactotetraosylceramide(H-D5) and NeuGc-lacto-N-norhexaosylceramide (H-D7) in all 5 melanoma extracts.