Vimentin is secreted by activated macrophages
Top Cited Papers
- 16 December 2002
- journal article
- letter
- Published by Springer Science and Business Media LLC in Nature
- Vol. 5 (1), 59-63
- https://doi.org/10.1038/ncb898
Abstract
Vimentin is a widely expressed intermediate filament protein thought to be involved mainly in structural processes, such as wound healing1. We now demonstrate that activated human macrophages secrete vimentin into the extracellular space. The maturation of blood-derived monocytes into macrophages involves several signalling pathways2,3. We show that secretion of vimentin, which is phosphorylated at serine and threonine residues, is enhanced by the phosphatase inhibitor okadaic acid and blocked by the specific protein kinase C inhibitor GÖ6983. These findings are consistent with previous observations that phosphorylation of vimentin affects its intracellular localization and that vimentin is a substrate for protein kinase C (PKC)4,5. We also show that the anti-inflammatory cytokine interleukin-10 (IL-10), which inhibits PKC activity, blocks secretion of vimentin. In contrast, the pro-inflammatory cytokine tumour necrosis factor α (TNF-α) can trigger secretion of vimentin. Finally, we found that extracellular vimentin is involved in bacterial killing and the generation of oxidative metabolites, two important functions of activated macrophages. These data establish that vimentin is secreted by macrophages in response to pro-inflammatory signalling pathways and is probably involved in immune function.Keywords
This publication has 26 references indexed in Scilit:
- Protein kinases required for segregation of vimentin filaments in mitotic processOncogene, 2001
- A Novel Interaction of the Golgi Complex with the Vimentin Intermediate Filament CytoskeletonThe Journal of cell biology, 2001
- Regulation of Elastinolytic Cysteine Proteinase Activity in Normal and Cathepsin K–Deficient Human MacrophagesThe Journal of Experimental Medicine, 2000
- Vimentin Dephosphorylation by Protein Phosphatase 2A Is Modulated by the Targeting Subunit B55Molecular Biology of the Cell, 1999
- Identification of mitogen-activated protein kinase-activated protein kinase-2 as a vimentin kinase activated by okadaic acid in 9L rat brain tumor cellsJournal of Cellular Biochemistry, 1998
- Pericellular mobilization of the tissue-destructive cysteine proteinases, cathepsins B, L, and S, by human monocyte-derived macrophages.Proceedings of the National Academy of Sciences of the United States of America, 1995
- The induction of vimentin gene expression by sodium butyrate in human promonocytic leukemia U937 cellsExperimental Cell Research, 1990
- Intermediate FilamentsPublished by Springer Science and Business Media LLC ,1985
- The cytoskeleton in activated and in functionally disordered cells of the macrophage systemPathology - Research and Practice, 1982
- Vimentin filaments in peritoneal macrophages at various stages of differentiation and with altered functionVirchows Archiv B Cell Pathology Including Molecular Pathology, 1982