Dendritic cell-based in vitro assays for vaccine immunogenicity
Open Access
- 16 September 2012
- journal article
- Published by Taylor & Francis Ltd in Human Vaccines & Immunotherapeutics
- Vol. 8 (9), 1323-1325
- https://doi.org/10.4161/hv.21350
Abstract
Dendritic cells (DC) are pivotal in the induction of adaptive immune responses because they can activate naive T-cells. Moreover, they steer these adaptive immune responses by integrating various stimuli, such as from different pathogen associated molecular patterns and the cytokine milieu. Immature DC are very well capable of ingesting protein antigens, whereas mature DC are efficient presenters of peptides to naive T cells. Human DC can be readily cultured from peripheral blood mononuclear cells, which are isolated from human blood. There is a strong need to monitor in a high-throughput fashion the immunogenicity of candidate vaccines during the process of vaccine development. Furthermore, regulators require efficacy and safety testing for batch release. For some vaccines, these tests require animal testing, causing pain and discomfort, which cannot be contested because it would interfere with the test results. With the aims of promoting vaccine development and reducing the number of animals for batch release testing, we propose to use more broadly human DC for vaccine immunogenicity testing. In this commentary, this proposition is illustrated by several examples in which the maturation of human DC was successfully used to test for vaccine and adjuvant immunogenicity.Keywords
This publication has 14 references indexed in Scilit:
- Response of MUTZ-3 dendritic cells to the different components of the Haemophilus influenzae type B conjugate vaccine: Towards an in vitro assay for vaccine immunogenicityVaccine, 2011
- Assessing Protein Immunogenicity with a Dendritic Cell Line-Derived Endolysosomal DegradomePLOS ONE, 2011
- The novel adjuvant CoVaccineHT™ increases the immunogenicity of cell-culture derived influenza A/H5N1 vaccine and induces the maturation of murine and human dendritic cells in vitroVaccine, 2009
- Transcriptional and functional defects of dendritic cells derived from the MUTZ-3 leukaemia lineImmunology, 2009
- IFN-β improves BCG immunogenicity by acting on DC maturationJournal of Leukocyte Biology, 2008
- Yellow fever vaccine YF-17D activates multiple dendritic cell subsets via TLR2, 7, 8, and 9 to stimulate polyvalent immunityThe Journal of Experimental Medicine, 2006
- Interferon regulatory factor 3 is involved in Toll-like receptor 4 (TLR4)- and TLR3-induced IL-12p35 gene activationBlood, 2006
- Impaired responses of leukemic dendritic cells derived from a human myeloid cell line to LPS stimulationExperimental & Molecular Medicine, 2006
- Functional and transcriptional profiling of MUTZ‐3, a myeloid cell line acting as a model for dendritic cellsImmunology, 2006
- Dendritic cells and the control of immunityNature, 1998