Early Assessment of Pulmonary Inflammation by 19 F MRI In Vivo
- 1 March 2010
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation: Cardiovascular Imaging
- Vol. 3 (2), 202-210
- https://doi.org/10.1161/circimaging.109.902312
Abstract
Background— Emulsified perfluorocarbons (PFCs) are preferentially phagocytized by monocytes/macrophages and are readily detected by 19F MRI. This study tests the hypothesis that 19F MRI can be used to quantitate pulmonary inflammation by tracking of infiltrating PFC-loaded monocytes. Methods and Results— Pneumonia was induced in mice by intratracheal instillation of lipopolysaccharides (LPS) followed by intravenous injection of PFCs. Whereas regular 1H MRI provided no evidence of lung injury 24 hours after LPS, the concurrent 19F images clearly show PFC accumulation in both pulmonary lobes. Imaging at 48 hours after LPS revealed signals in 1H images at the same location as the 24-hour 19F signals. Thus, progressive pneumonia was first predicted by 19F MRI early after PFC administration. Without LPS, at no time were 19F signals observed within the lung. Histology and fluorescence-activated cell sorting (FACS) combined with 19F MRI confirmed the presence of infiltrating PFC-loaded monocytes/macrophages after LPS challenge. Additional experiments with graded doses of LPS demonstrated that 19F signal intensity strongly correlated with both LPS dose and pathological markers of lung inflammation. In separate studies, dexamethasone and CGS21680 (adenosine 2A receptor agonist) were used to demonstrate the ability of 19F MRI to monitor anti-inflammatory therapies. Conclusions— PFCs serve as a contrast agent for the prognostic and quantitative assessment of pulmonary inflammation by in vivo 19F MRI, which is characterized by a high degree of specificity due to the lack of any 19F background. Because PFCs are biochemically inert, this approach may also be suitable for human applications.This publication has 35 references indexed in Scilit:
- Plasma C-Reactive Protein Levels Are Associated With Improved Outcome in ARDSSocial psychiatry. Sozialpsychiatrie. Psychiatrie sociale, 2009
- Cellular compartmentalization of internalized paramagnetic liposomes strongly influences both T1and T2relaxivityMagnetic Resonance in Medicine, 2009
- The cytokine network in asthma and chronic obstructive pulmonary diseaseJCI Insight, 2008
- In Vivo Monitoring of Inflammation After Cardiac and Cerebral Ischemia by Fluorine Magnetic Resonance ImagingCirculation, 2008
- Extending the Frontiers of Cardiac Magnetic ResonanceCirculation, 2008
- Procalcitonin is a valuable prognostic marker in ARDS caused by community‐acquired pneumoniaRespirology, 2008
- Alveolar macrophages contribute to alveolar barrier dysfunction in ventilator-induced lung injuryAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2006
- Adenosine A2A receptor activation reduces lung injury in trauma/hemorrhagic shock*Critical Care Medicine, 2006
- 1H MRI of pneumococcal pneumonia in a murine modelJournal of Magnetic Resonance Imaging, 2005
- Fast proton spectroscopic imaging of human brain using multiple spin‐echoesMagnetic Resonance in Medicine, 1993