Epidemiological description and analysis of RdRp, E and N genes dynamic by RT-PCR of SARS-CoV-2 in Moroccan population: Experience of the National Reference Laboratory (LNR)-UM6SS
Open Access
- 20 June 2020
- preprint content
- other
- Published by Cold Spring Harbor Laboratory
Abstract
The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a new infectious disease that first emerged in Hubei province, China, in December 2019. On 2 March 2020, the Moroccan Ministry of Health confirmed the first COVID-19 case in Morocco. The new virus SARS-CoV-2 was identified in the sample of a Moroccan expatriate residing in Italy. Without a therapeutic vaccine or specific antiviral drugs, early detection and isolation become essential against novel Coronavirus.This study aims to analyze the epidemiological profile of the SARS-CoV-2 in Moroccan cases and to investigate the dynamic of RdRp, N, and E genes in patients from diagnosis until the recovery.Among 859 COVID-19 RT-PCR tests realized for 376 patients, 187 cases had positive results COVID-19. 4% were positive with the 3 genes RdRp, N, and E, 40 % with N and E genes, 3% with RdRp and N genes, 31% with only the RdRp gene and 22% cases are positives with N gene. The analysis of the Covid-19 genes (RdRp, N, and E) dynamic reveal that more than 6% stay positive with detection of the N and E gene, and 14% with the N gene after 12 days of treatment.The median period from positive to the first negative Covid-19 RT-PCR tests was 6.8±2.24 days for 44% cases, 14.31± 2.4 days for 30%, and 22.67 ± 1.21 days for 4%.This a first description of the Moroccan COVID-19 cases and the analysis of the dynamic of the RdRp, N, and E genes. The analysis of our population can help to improve in the care of patients.Keywords
This publication has 12 references indexed in Scilit:
- Presumed Asymptomatic Carrier Transmission of COVID-19Jama-Journal Of The American Medical Association, 2020
- SARS-CoV-2 Viral Load in Upper Respiratory Specimens of Infected PatientsThe New England Journal of Medicine, 2020
- Asians Do Not Exhibit Elevated Expression or Unique Genetic Polymorphisms for ACE2, the Cell-Entry Receptor of SARS-CoV-2Published by MDPI AG ,2020
- A pneumonia outbreak associated with a new coronavirus of probable bat originNature, 2020
- Clinical features of patients infected with 2019 novel coronavirus in Wuhan, ChinaThe Lancet, 2020
- Prevalence of comorbidities in the Middle East respiratory syndrome coronavirus (MERS-CoV): a systematic review and meta-analysisInternational Journal of Infectious Diseases, 2016
- The Coronavirus Nucleocapsid Is a Multifunctional ProteinViruses, 2014
- Antagonism of the Interferon-Induced OAS-RNase L Pathway by Murine Coronavirus ns2 Protein Is Required for Virus Replication and Liver PathologyCell Host & Microbe, 2012
- siRNA targeting the Leader sequence of SARS-CoV inhibits virus replicationGene Therapy, 2005
- The Genome Sequence of the SARS-Associated CoronavirusScience, 2003