Prospective Study of Quantitation of Plasma DNA Levels in the Diagnosis of Malignant versus Benign Prostate Disease
- 15 February 2005
- journal article
- research article
- Published by American Association for Cancer Research (AACR) in Clinical Cancer Research
- Vol. 11 (4), 1394-1399
- https://doi.org/10.1158/1078-0432.ccr-04-1237
Abstract
Purpose: The aim of this study was to determine the potential of cell-free DNA levels as a diagnostic marker for prostate cancer, having first established the effect that blood sample processing has on this measurement. Experimental Design: A total of 152 blood samples were collected prospectively from patients before their prostate biopsy and 25 from men in two distinct control groups. Blood was processed to yield three components: one-spin plasma (n = 68), two-spin plasma (n = 152), and serum (n = 56) samples. Results: Having established the effect of sample preparation on the measured DNA level, the more reliable two-spin plasma sample was used to determine the relationship between DNA and the presence of prostate cancer. Those patients with cancer (n = 78) had a significantly higher level of DNA compared with the control groups (P < 0.0001 and P < 0.0001). However, DNA levels in patients with a benign biopsy (n = 74) were significantly higher than the 78 patients confirmed to have cancer (P = 0.02). Conclusions: We conclude that the sample type used in the quantitation of cell-free DNA has an effect on the level reported. Elevated levels are present in the two-spin plasma samples of patients with prostate cancer compared with healthy controls but are not of diagnostic value during the management of prostate cancer.Keywords
This publication has 19 references indexed in Scilit:
- Intra-individual variation of serum prostate specific antigen levels in men with benign prostate biopsiesBJU International, 2004
- Quantification of Free Circulating DNA As a Diagnostic Marker in Lung CancerJournal of Clinical Oncology, 2003
- Amplification of HSD17B1 and ERBB2 in primary breast cancerOncogene, 2003
- INCIDENCE AND CLINICAL SIGNIFICANCE OF FALSE-NEGATIVE SEXTANT PROSTATE BIOPSIESJournal of Urology, 1998
- Quantitative Analysis of Fetal DNA in Maternal Plasma and Serum: Implications for Noninvasive Prenatal DiagnosisAmerican Journal of Human Genetics, 1998
- Prevalence and Predictors of a Positive Repeat Transrectal Ultrasound Guided Needle Biopsy of the ProstateJournal of Urology, 1997
- Biological Variation of Total, Free and Percent Free Serum Prostate Specific Antigen Levels in Screening VolunteersJournal of Urology, 1997
- Prostate cancer detection in men with serum PSA concentrations of 2.6 to 4.0 ng/mL and benign prostate examination. Enhancement of specificity with free PSA measurementsPublished by American Medical Association (AMA) ,1997
- Effect of Inflammation and Benign Prostatic Hyperplasia on Elevated Serum Prostate Specific Antigen LevelsJournal of Urology, 1995
- Neoplastic Characteristics of the DNA Found in the Plasma of Cancer PatientsOncology, 1989