Identification of the NH2‐terminal blocking group of calcineurin B as myristic acid
Open Access
- 27 December 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 150 (2), 314-318
- https://doi.org/10.1016/0014-5793(82)80759-x
Abstract
The NH2‐terminal blocking group of the Ca 2+‐binding B‐subunit of calcineurin (protein phosphatase‐2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl‐Gly‐Asn‐Glu‐Ala‐, is very similar to that of the catalytic subunit of cyclic AMP‐dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.Keywords
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