Spectrofluorimetric and Spectrophotometric Determination of Irbesartan and Bisoprolol hemifumarate independently in their Tablets

Abstract

Sensitive, simple and rapid spectrofluorimetric and spectrophotometric methods were developed for the determination of Irbesartan (IRB) and Bisoprolol hemifumarate (BPH) in their tablets dosage form. Both methodologies are based on charge transfer reaction between the studied drugs and 7-Chloro-4-nitrobenzen-2-oxa-1, 3-diazole NBD-Cl. The developed orange products were measured in the appropriate solvent fluorometrically at 534 and 538 nm after excitation at 465 and 470 nm for IRB and BPH, respectively. The fluorescence–concentration plots were rectilinear over the range of 2.5–8 μg/mL for IRB and 6–16 μg/mL for BPH with lower detection limits of 0.18 and 0.39 μg/mL and a lower quantification limit of 0.55 and 1.17 μg/mL for IRB and BPH, respectively. The spectrophotometric method is based on measuring the orange colored products which were developed upon charge transfer complex formation between the studied drugs and (NBD-Cl) in organic media. This showed absorption maxima at 476 nm and 479 nm for IRB and BPH, respectively. The absorbance–concentration plots were rectilinear over the range of 20–90 μg/mL for IRB and 40–160 μg/mL for BPH with lower detection limits of 1.37 and 3.98 μg/mL and lower quantification limits of 4.17 and 12.06 μg/mL for IRB and BPH, respectively. Both methods were successfully applied to the analysis of the two selected drugs. The current study showed that the results were in good agreement with the reference methods.