Impact of the Placental Cytokine-Chemokine Balance on Regulation of Cell-Cell Contact-Induced Human Immunodeficiency Virus Type 1 Translocation across a Trophoblastic Barrier In Vitro
Open Access
- 1 October 2005
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 79 (19), 12304-12310
- https://doi.org/10.1128/jvi.79.19.12304-12310.2005
Abstract
Cells constituting the placental barrier secrete soluble factors that may participate in controlling human immunodeficiency virus type 1 (HIV-1) transmission from the mother to the fetus. In this study, we asked whether placental soluble factors (PSF) could limit cell-cell contact inducing HIV-1 production that occurs after inoculation of HIV-1-infected peripheral blood mononuclear cells (HIV-1+PBMCs) onto trophoblast-derived BeWo cells grown as tight and polarized barriers in a two-chamber system. The activity of recombinant chemokines and cytokines expressed by placental tissue and of factors secreted by either early or term placentae of HIV-1-negative women, was analyzed. We identified chemokines (RANTES and MIP-1β) and cytokines (tumor necrosis factor alpha and interleukin-8) that decreased and increased, respectively, viral production in trophoblast barrier cells inoculated with HIV-1+PBMCs. Unexpectedly, factors secreted by either early or term placentae of HIV-1-negative women enhanced viral production. Nevertheless, the same PSF did not favor infection of trophoblastic barriers with cell-free HIV-1 and strongly reduced viral production in PBMCs infected with cell-free HIV-1. Moreover, PSF contained chemokines (RANTES and MIP-1β) and a cytokine, leukemia inhibitory factor, exhibiting a strong anti-HIV-1 activity in our model of cell-to-cell infection. Together these data suggested that at the maternal interface the global activity of PSF is related to the synergistic action of several soluble factors with a balance in favor of an enhancing activity on the passage of viruses across the trophoblast barrier. This could explain the presence of viral sequences in trophoblasts in all placentae of HIV-1-infected women.Keywords
This publication has 29 references indexed in Scilit:
- Changes in the Levels of Chemokines and Cytokines in the Placentas of Women withPlasmodium falciparumMalariaThe Journal of Infectious Diseases, 2003
- Cell-Free HIV Type 1 Infection is Restricted in the Human Trophoblast Choriocarcinoma BeWo Cell Line, Even with Expression of CD4, CXCR4 and CCR5AIDS Research and Human Retroviruses, 2003
- Role of Placental Cytokines in Transcriptional Modulation of HIV Type 1 in the Isolated Villous TrophoblastAIDS Research and Human Retroviruses, 2002
- Twenty-four month efficacy of a maternal short-course zidovudine regimen to prevent mother-to-child transmission of HIV-1 in West AfricaAIDS, 2002
- Extinguishing maternal immune responses during pregnancy: implications for immunosuppressionSeminars in Immunology, 2001
- Up-Regulation of CCR5 Expression in the Placenta Is Associated with Human Immunodeficiency Virus-1 Vertical TransmissionThe American Journal of Pathology, 2000
- Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8 + T CellsScience, 1995
- HIV suppression by interleukin-16Nature, 1995
- Cell-to-cell transmission of human immunodeficiency virus infection induces two distinct phases of viral RNA expression under separate regulatory controlJournal of General Virology, 1993
- Cell-Mediated Infection of Human Placental Trophoblast with HIV In VitroAIDS Research and Human Retroviruses, 1991