The RosR transcription factor is required for gene expression dynamics in response to extreme oxidative stress in a hypersaline-adapted archaeon
Open Access
- 30 July 2012
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Genomics
- Vol. 13 (1), 351
- https://doi.org/10.1186/1471-2164-13-351
Abstract
Background: Previous work has shown that the hypersaline-adapted archaeon, Halobacterium salinarum NRC-1, is highly resistant to oxidative stress caused by exposure to hydrogen peroxide, UV, and gamma radiation. Dynamic alteration of the gene regulatory network (GRN) has been implicated in such resistance. However, the molecular functions of transcription regulatory proteins involved in this response remain unknown. Results: Here we have reanalyzed several existing GRN and systems biology datasets for H. salinarum to identify and characterize a novel winged helix-turn-helix transcription factor, VNG0258H, as a regulator required for reactive oxygen species resistance in this organism. This protein appears to be unique to the haloarchaea at the primary sequence level. High throughput quantitative growth assays in a deletion mutant strain implicate VNG0258H in extreme oxidative stress resistance. According to time course gene expression analyses, this transcription factor is required for the appropriate dynamic response of nearly 300 genes to reactive oxygen species damage from paraquat and hydrogen peroxide. These genes are predicted to function in repair of oxidative damage to proteins and DNA. In vivo DNA binding assays demonstrate that VNG0258H binds DNA to mediate gene regulation. Conclusions: Together these results suggest that VNG0258H is a novel archaeal transcription factor that regulates gene expression to enable adaptation to the extremely oxidative, hypersaline niche of H. salinarum. We have therefore renamed VNG0258H as RosR, for r eactive o xygen s pecies r egulator.This publication has 58 references indexed in Scilit:
- Identification and Genomic Analysis of Transcription Factors in Archaeal Genomes Exemplifies Their Functional Architecture and Evolutionary OriginMolecular Biology and Evolution, 2010
- Coordination of frontline defense mechanisms under severe oxidative stressMolecular Systems Biology, 2010
- Prevalence of transcription promoters within archaeal operons and coding sequencesMolecular Systems Biology, 2009
- A single transcription factor regulates evolutionarily diverse but functionally linked metabolic pathways in response to nutrient availabilityMolecular Systems Biology, 2009
- Halobacterium salinarum NRC-1 PeptideAtlas: Toward Strategies for Targeted Proteomics and Improved Proteome CoverageJournal of Proteome Research, 2008
- Genome information management and integrated data analysis with HaloLexArchiv für Mikrobiologie, 2008
- Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegansNature Protocols, 2008
- General transcription factor specified global gene regulation in archaeaProceedings of the National Academy of Sciences of the United States of America, 2007
- MEME: discovering and analyzing DNA and protein sequence motifsNucleic Acids Research, 2006
- An integrated systems approach for understanding cellular responses to gamma radiationMolecular Systems Biology, 2006