Fc-Epsilon-RI, the High Affinity IgE-Receptor, Is Robustly Expressed in the Upper Gastrointestinal Tract and Modulated by Mucosal Inflammation
Open Access
- 27 July 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (7), e42066
- https://doi.org/10.1371/journal.pone.0042066
Abstract
The role of the high affinity IgE receptor, FcεRI, in IgE-mediated immune responses of the gastrointestinal (GI) mucosa is poorly understood. Currently, a detailed characterization of FcεRI expression throughout the human gut is lacking. The aim of this study was to define the expression pattern of FcεRI in the GI tract. We compared FcεRI expression in children with gastritis/esophagitis (n = 10), celiac disease (n = 10), inflammatory bowel disease (IBD) (n = 9), and normal mucosa (n = 5). The α–subunit of FcεRI (FcεRIα), detected by immunohistochemistry, was found on cells infiltrating the mucosa of the esophagus, the stomach, and the duodenum, but was rarely detected in more distal sections of the GI tract. Accordingly, quantitative RT-PCR analysis on esophagus, stomach, duodenum, colon, and rectum biopsies revealed that FcεRIα and -β expression levels decreased towards the distal intestine. mRNA transcripts of the common Fc-receptor-γ chain were present in the entire GI mucosa. Double-immunofluorescence staining of esophageal specimens confirmed that FcεRIα was expressed on intraepithelial mast cells and Langerhans cells. The mRNA expression levels of the α, β, and γ subunits of FcεRI did not correlate with total serum IgE but were associated with mucosal inflammation. Our data define the upper GI tract as the main site for IgE-mediated immune activation via FcεRI. Tissue mRNA levels of FcεRIα are regulated by inflammatory conditions rather than serum IgE, indicating that FcεRI might also play a role in pathologies other than allergy.This publication has 38 references indexed in Scilit:
- Expression of High-Affinity IgE Receptor on Human Peripheral Blood Dendritic Cells in ChildrenPLOS ONE, 2012
- Comparative Analysis of FcεRI Expression Patterns in Patients With Eosinophilic and Reflux EsophagitisJournal of Pediatric Gastroenterology and Nutrition, 2010
- Relationships between Levels of Serum IgE, Cell-Bound IgE, and IgE-Receptors on Peripheral Blood Cells in a Pediatric PopulationPLOS ONE, 2010
- The High Affinity IgE Receptor FcεRI Is Expressed by Human Intestinal Epithelial CellsPLOS ONE, 2010
- Local B cells and IgE production in the oesophageal mucosa in eosinophilic oesophagitisGut, 2009
- Allergen-IgE Complexes Trigger CD23-Dependent CCL20 Release From Human Intestinal Epithelial CellsGastroenterology, 2007
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Mast cells from human gastric mucosa: A comparative study with lung and colonic mast cellsInflammation Research, 1991
- Fc ReceptorsAnnual Review of Immunology, 1991
- IgE-Positive Cells in Human Intestinal Mucosa Are Mainly Mast CellsInternational Archives of Allergy and Immunology, 1989