Localization of IL-1, IL-2, IL-4, IL-8 and TNF in Superficial Bladder Tumors Treated with Intravesical Bacillus Calmette-Guerin
- 1 August 1996
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of Urology
- Vol. 156, 536-541
- https://doi.org/10.1097/00005392-199608000-00078
Abstract
Cytokines have been detected in the urine during the first hours after intravesical Bacillus Calmette Guerin (BCG) treatment against superficial bladder cancer. To investigate the long-lasting mucosal inflammatory response, we analyzed intracellular cytokines by immunohistochemistry in biopsies taken 2 weeks after BCG treatment. Tumor biopsies were obtained from 8 patients with noninvasive, papillary transitional cell carcinoma (TCC), and intracellular cytokines were visualized by immunohistochemistry using cytokine-specific monoclonal antibodies. Interleukin (IL)-1 beta+ or tumor necrosis factor (TNF)-alpha+ cells were abundant in tumor and stroma. Interleukin-1 alpha, IL-2, IL-4, IL-8 and TNF-beta were variably expressed, while IL-10+ and interferon (IFN)-gamma+ cells were not detected. Among the few patients studied (5 responders and 3 nonresponders to BCG treatment) no single cytokine or cytokine profile was associated with clinical response to BCG therapy. We conclude that the in situ cytokine response after BCG treatment is highly complex, since cytokine profiles differed among the 8 patients investigated and between tumor and surrounding tumor-free mucosa. Further studies, investigating larger numbers of patients, is required to clarify whether cytokine profiles correlate with the clinical response to BCG.Keywords
This publication has 15 references indexed in Scilit:
- Lipopolysaccharide induces human interleukin‐1 receptor antagonist and interleukin‐1 production in the same cellEuropean Journal of Immunology, 1992
- Induction of urinary interleukin-1 (IL-1), IL-2, IL-6, and tumour necrosis factor during intravesical immunotherapy with bacillus Calmette-Guérin in superficial bladder cancerCancer Immunology, Immunotherapy, 1992
- Detection of interleukin 8 and tumor necrosis factor in normal humans after intravenous endotoxin: the effect of antiinflammatory agents.The Journal of Experimental Medicine, 1991
- Assessment of Cytokines by Immunofluorescence and the Paraformaldehyde‐Saponin ProcedureImmunological Reviews, 1991
- Neutrophil-Activating Peptide-1/Interleukin-8 Detection in Human Urine during Acute Bladder Inflammation Caused by Transurethral Resection of Superficial Cancer and Bacillus Calmette-Guérin AdministrationEuropean Urology, 1991
- Highly sensitive enzyme immunoassays for antibodies to human tumor necrosis factor (TNF-α) and lymphotoxin (TNF-β)Journal of Immunological Methods, 1990
- Elevations of Cytokines Interleukin-1, Interleukin-2 and Tumor Necrosis Factor in the Urine of Patients after Intravesical Bacillus Calmette-Guerin ImmunotherapyJournal of Urology, 1990
- Urinary interleukins in patients receiving intravesical bacillus calmette-guerin therapy for superficial bladder cancerCancer, 1989
- Detection of Interleukin 2 in the Urine of Patients with Superficial Bladder Tumors after Treatment with Intravesical BCGJournal of Urology, 1986
- Intracavitary Bacillus Calmette-guerin in the Treatment of Superficial Bladder TumorsJournal of Urology, 1976