Chlorophyll fluorescence as a probe for the determination of the photo-induced proton gradient in isolated chloroplasts

Abstract
Gramicidin D-treated chloroplasts show an acid-induced quenching of the chlorophyll fluorescence, which is composed of a reversible and irreversible part. The reversible quenching is analogous to the photo-induced quenching in coupled chloroplasts and can be taken to determine the light induced ΔpH.

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