Real-Time Monitoring Surface Chemistry-Dependent In Vivo Behaviors of Protein Nanocages via Encapsulating an NIR-II Ag2S Quantum Dot
- 28 October 2015
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Nano
- Vol. 9 (12), 12255-12263
- https://doi.org/10.1021/acsnano.5b05503
Abstract
Protein nanocages (PNCs) have been recognized as a promising platform for nanomedicine innovation. Real-time in vivo tracking of PNCs can provide critically important information for the development of PNC-based diagnostics and therapeutics. Here we demonstrate a general strategy for monitoring the behaviors of PNCs in vivo by encapsulating a Ag2S quantum dot (QD) with fluorescence in the second near-infrared window (NIR-II, 1000-1700 nm) inside the PNC, using simian virus 40 (SV40) PNC (PNCSV40) as a model. Benefiting from the high spatiotemporal resolution and deep tissue penetration of NIR-II fluorescence imaging, the dynamic distribution of the PNCSV40 in living mice was tracked in real time with high fidelity, and adopting the PEGylation strategy, surface chemistry-dependent in vivo behaviors of PNCSV40 were clearly revealed. This study represents the first evidence of real-time tracking of the intrinsic behaviors of PNCs in vivo without interference in PNC-host interactions by encapsulating nanoprobes inside. The as-described imaging strategy will facilitate the study of interactions between exogenously introduced PNCs and host body and prompt the development of future protein-based drugs, sensors, and high-efficacy targeted delivery systems.Keywords
Funding Information
- Chinese Academy of Sciences (KGZD-EW-T02-3, XDA01030200)
- Ministry of Science and Technology of the People's Republic of China (2011CB965004)
- National Natural Science Foundation of China (21303249, 21425103, 21501192, 31271076, 31470931, 81401464)
- Natural Science Foundation of Jiangsu Province (BK2012007, BK20130366)
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