Pep5, a new lantibiotic: structural gene isolation and prepeptide sequence
- 1 June 1989
- journal article
- research article
- Published by Springer Science and Business Media LLC in Archiv für Mikrobiologie
- Vol. 152 (1), 16-19
- https://doi.org/10.1007/bf00447005
Abstract
A wobbled 14-mer oligonucleotide was derived from the amino acid sequence of the 34-residue propeptide of the lantibiotic Pep5 (Kellner et al. 1989). Using this hybridization probe, the structural gene of Pep5, pepA, was located on the 18.6 kbp plasmid pED503. The nucleotide sequence of pepA codes for a prepeptide with 60 residues and proves that Pep5 is ribosomally synthesized. The N-terminus of the prepeptide has a high α-helix probability and a characteristic proteolytic cleavage site precedes the C-terminal 34-residue propeptide. Our present theory is that maturation of Pep5 involves (a) enzymic conversion of Thr, Ser and Cys into dehydrated amino acids and sulfide bridges, (b) membrane translocation and cleavage of the modified prepeptide.Keywords
This publication has 31 references indexed in Scilit:
- Different mechanisms of insensitivity to the staphylococcin-like peptide pep 5Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology, 1987
- Epidermin: sequencing of a heterodet tetracyclic 21‐peptide amide antibioticJBIC Journal of Biological Inorganic Chemistry, 1986
- Elucidation of the Structure of Epidermin, a Ribosomally Synthesized, Tetracyclic Heterodetic Polypeptide AntibioticAngewandte Chemie, 1985
- Prediction of chain flexibility in proteinsThe Science of Nature, 1985
- Plasmid Involvement in Production of and Immunity to the Staphylococcin-like Peptide Pep 5Microbiology, 1984
- A computer program for predicting protein antigenic determinantsMolecular Immunology, 1983
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteinsJournal of Molecular Biology, 1978
- Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gelsJournal of Molecular Biology, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975