Sequence of the coding regions from the 3.0 kb and 3.9 kb mRNA
- 1 September 1989
- journal article
- Published by Springer Science and Business Media LLC in Archiv für die gesamte Virusforschung
- Vol. 105 (3-4), 165-178
- https://doi.org/10.1007/bf01311354
Abstract
Subgenomic mRNA from a virulent isolate of porcine transmissible gastroenteritis virus (TGEV) was used to produce cDNA clones covering the genome region from the 3′ end of the pelomer gene to the start of the integral membrane protein gene. The nucleotide sequence of this area was determined using clone pTG11 and a previously reported cDNA clone pTG22. Three open reading frames (ORFs) were identified encoding putative polypeptides of relative molecular masses (Mr) 6,600, 27,600, and 9,200. The sequence encoding the Mr 9,200 polypeptide was found to be present on the “unique” 5′ region of the 3.0 kb mRNA species whereas the other two ORFs mapped on the 3.9 kb mRNA species. Differences between the ORFs from this strain of TGEV and those from a previously reported avirulent strain of TGEV were compared.Keywords
This publication has 37 references indexed in Scilit:
- The integral membrane protein from a virulent isolate of transmissible gastroenteritis virus: molecular characterization, sequence and expression in Escherichia coliMolecular Microbiology, 1988
- Identification of the Coronavirus MHV-JHM mRNA 4 ProductJournal of General Virology, 1988
- Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomesCell, 1986
- Sequencing of Coronavirus IBV Genomic RNA: Three Open Reading Frames in the 5' 'Unique' Region of mRNA DJournal of General Virology, 1985
- Rapid and Sensitive Protein Similarity SearchesScience, 1985
- Coding Sequence of Coronavirus MHV-JHM mRNA 4Journal of General Virology, 1985
- Coronavirus MHV-JHM mRNA 5 Has a Sequence Arrangement which Potentially Allows Translation of a Second, Downstream Open Reading FrameJournal of General Virology, 1985
- A Leader Sequence is Present on mRNA A of Avian Infectious Bronchitis VirusJournal of General Virology, 1984
- Location and Direction of Transcription of the ptsH and ptsI Genes on the Escherichia coli K12 GenomeMicrobiology, 1984
- The Polypeptide Structure of Transmissible Gastroenteritis VirusJournal of General Virology, 1975