Spatial and temporal tracking of cardiac exosomes in mouse using a nano-luciferase-CD63 fusion protein
Open Access
- 10 March 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Communications Biology
- Vol. 3 (1), 1-9
- https://doi.org/10.1038/s42003-020-0830-7
Abstract
Exosomes are secreted extracellular vesicles with lipid bilayer membranes. They are emerging as a new category of messengers that facilitate cross-talk between cells, tissues, and organs. Thus, a critical demand arises for the development of a sensitive and non-invasive tracking system for endogenous exosomes. We have generated a genetic mouse model that meets this goal. The Nano-luciferase (NanoLuc) reporter was fused with the exosome surface marker CD63 for exosome labeling. The cardiomyocyte-specific αMHC promoter followed by the loxP-STOP-loxP cassette was engineered for temporal and spatial labeling of exosomes originated from cardiomyocytes. The transgenic mouse was bred with a tamoxifen-inducible Cre mouse (Rosa26Cre-ERT2) to achieve inducible expression of CD63NanoLuc reporter. The specific labeling and tissue distribution of endogenous exosomes released from cardiomyocytes were demonstrated by luciferase assay and non-invasive bioluminescent live imaging. This endogenous exosome tracking mouse provides a useful tool for a range of research applications.Keywords
Funding Information
- American Heart Association (18IPA34180012)
This publication has 56 references indexed in Scilit:
- Comparative marker analysis of extracellular vesicles in different human cancer typesJournal of Extracellular Vesicles, 2013
- Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone SubstrateACS Chemical Biology, 2012
- Exosomes From Human CD34 + Stem Cells Mediate Their Proangiogenic Paracrine ActivityCirculation Research, 2011
- A Novel Nanoparticle Drug Delivery System: The Anti-inflammatory Activity of Curcumin Is Enhanced When Encapsulated in ExosomesMolecular Therapy, 2010
- Rab27a and Rab27b control different steps of the exosome secretion pathwayNature, 2009
- Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivoNature Protocols, 2009
- Sensitive in vivo imaging of T cells using a membrane-bound Gaussia princeps luciferaseNature Medicine, 2009
- Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cellsNature, 2007
- Exosome Display technology: Applications to the development of new diagnostics and therapeuticsBlood Cells, Molecules, and Diseases, 2005
- Codon-Optimized Gaussia Luciferase cDNA for Mammalian Gene Expression in Culture and in VivoMolecular Therapy, 2005