Cloning of chromosomal β-lactamase genes from Yersinia enterocolitica

Abstract
Summary: Two β-lactamase genes present in the chromosome of Yersinia enterocolitica have been cloned individually into the plasmid pACY184 and expressed in Escherichia coli. The gene for broad-spectrum β-lactamase I (‘A’) was cloned from a strain belonging to the O:3 serotype, and the gene for (cephalosporinase) β-lactomase II (‘B’) was cloned from a strain of the O:5b serotype. The properties of the β-lactamases expressed in E. coli are similar to those previously described in Y. enterocolitica.