Identification of the Nucleocapsid, Tegument, and Envelope Proteins of the Shrimp White Spot Syndrome Virus Virion
Open Access
- 15 March 2006
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 80 (6), 3021-3029
- https://doi.org/10.1128/jvi.80.6.3021-3029.2006
Abstract
The protein components of the white spot syndrome virus (WSSV) virion have been well established by proteomic methods, and at least 39 structural proteins are currently known. However, several details of the virus structure and assembly remain controversial, including the role of one of the major structural proteins, VP26. In this study, Triton X-100 was used in combination with various concentrations of NaCl to separate intact WSSV virions into distinct fractions such that each fraction contained envelope and tegument proteins, tegument and nucleocapsid proteins, or nucleocapsid proteins only. From the protein profiles and Western blotting results, VP26, VP36A, VP39A, and VP95 were all identified as tegument proteins distinct from the envelope proteins (VP19, VP28, VP31, VP36B, VP38A, VP51B, VP53A) and nucleocapsid proteins (VP664, VP51C, VP60B, VP15). We also found that VP15 dissociated from the nucleocapsid at high salt concentrations, even though DNA was still present. These results were confirmed by CsCl isopycnic centrifugation followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and liquid chromatography-nanoelectrospray ionization-tandem mass spectrometry, by a trypsin sensitivity assay, and by an immunogold assay. Finally, we propose an assembly process for the WSSV virion.Keywords
This publication has 33 references indexed in Scilit:
- Identification and characterization of a prawn white spot syndrome virus gene that encodes an envelope protein VP31Virology, 2005
- A novel envelope protein involved in White spot syndrome virus infectionJournal of General Virology, 2005
- The Unique Stacked Rings in the Nucleocapsid of the White Spot Syndrome Virus Virion Are Formed by the Major Structural Protein VP664, the Largest Viral Structural Protein Ever FoundJournal of Virology, 2005
- Identification of a collagen-like protein gene from white spot syndrome virusArchiv für die gesamte Virusforschung, 2003
- Characterization of a novel envelope protein (VP281) of shrimp white spot syndrome virus by mass spectrometryJournal of General Virology, 2002
- Proteomic Analysis of Shrimp White Spot Syndrome Viral Proteins and Characterization of a Novel Envelope Protein VP466Molecular & Cellular Proteomics, 2002
- Identification of a Nucleocapsid Protein (VP35) Gene of Shrimp White Spot Syndrome Virus and Characterization of the Motif Important for Targeting VP35 to the Nuclei of Transfected Insect CellsVirology, 2002
- Major viral diseases of the black tiger prawn (Penaeus monodon) in ThailandWorld Journal of Microbiology and Biotechnology, 1997
- Ultrastructure and morphogenesis of White Spot Syndrome Baculovirus (WSSV)Diseases of Aquatic Organisms, 1997
- White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropodsDiseases of Aquatic Organisms, 1996